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1)  Multiplex(Duplex) PCR
多重(二重)PCR
2)  two-temperature multiplex RT-PCR
二温式多重RT-PCR
1.
Development of two-temperature multiplex RT-PCR technique for detecting two viruses on orchid;
二温式多重RT-PCR同时检测两种主要兰花病毒的研究
3)  two-temperature and multiple PCR
二温式多重PCR
1.
In the process of detection, a new modified two-temperature and multiple PCR was developed from three temperature and regular PCR.
根据多重PCR的技术原理,利用对虾(Penaeus vannamei)白斑综合征病毒和桃拉综合征病毒的基因序列分别设计了两对特异引物,并将常规三温式PCR扩增程序简化为2个温度梯度,建立二温式多重PCR技术用于对虾白斑综合征病毒(White spot syndrome virus,WSSV)和桃拉综合征病毒(Taura syndrome virus,TSV)的复合快速检测。
4)  multiplex PCR
多重PCR
1.
Detection of Salmonella spp.,Escherichia coli and Staphylococcus aureus by Multiplex PCR;
沙门菌、大肠杆菌和金黄色葡萄球菌的多重PCR检测
2.
Simultaneous Detection of Common Food-borne Bacterial Pathogens in Innocuous Poultry and Seafood by Multiplex PCR Assay;
多重PCR检测无公害畜禽肉和水产品中4种致病菌
3.
The clinical application of multiplex PCR in diagonsis of deafness with mtDNA mutation;
多重PCR诊断线粒体突变聋病的临床应用价值
5)  Multiplex polymerase chain reaction
多重PCR
1.
Multiplex polymerase chain reaction assay for sex determination from a single cell.;
应用单细胞多重PCR进行性别鉴定
2.
Objective To develop a novel multiplex polymerase chain reaction (PCR) to detect multidrug-resistant Acinetobacter baumannii.
目的建立一种适于临床微生物实验室快速检测多耐药鲍曼不动杆菌基因的多重PCR方法。
3.
A multiplex polymerase chain reaction(PCR)-based method was designed for simultaneous detection of four main viruses involved in respiratory and/or reproductive failure in piglets(porcine circovirus type 2 ,porcine parvovirus ,porcine reproductive and respiratory syndrome virus ,and classical swine fever virus ).
本试验建立一种可同时检测猪圆环病毒2型(PCV-2),猪细小病毒(PPV),猪繁殖与呼吸综合征病毒(PRRSV),猪瘟病毒(CSFV)4种病毒的多重PCR方法。
6)  multiple PCR
多重PCR
1.
Detection of hMSH2 gene mutations in gynecologic tumor population by multiple PCR-SSCP;
多重PCR-SSCP法检测妇科肿瘤患者hMSH2基因突变
2.
Detection of Herpes simplex Virus,Treponema pallidum and Haemophilus ducreyi by Multiple PCR
多重PCR同时检测苍白密螺旋体、单纯疱疹病毒及杜氏嗜血菌的实验研究
3.
Objective Using multiple PCR to screen the mutational status of the protease serine 1(PRSS1) gene and serine protease inhibitor Kazal type1(SPINK1) gene in chronic pancreatitis.
目的探讨多重PCR技术在筛查慢性胰腺炎患者的易感基因即胰蛋白酶原基因(protease serine 1,PRSS1)和胰腺囊性纤维化基因(serine protease inhibitor Kazal type1,SPINK1)突变的情况。
补充资料:二重曼荼罗
【二重曼荼罗】
 (术语)胎金两部之曼荼罗,各有浅略秘密之两重。在胎藏界者,大日经具缘品所说,大日住阿弥陀三摩地所现之加持境界也。是于三部中为莲华部之曼荼罗,浅略曼荼罗也。疏六曰:“青色是无量寿色,既到金刚实际即以加持方便,普现大悲曼荼罗,如净虚空中具含万德故。”又秘密品所说者,为毗卢遮那本地之境界,三部中之佛部曼荼罗即是秘密曼荼罗也。在金刚界者,教王经,略出经所说之大日,住阿閦三摩地,诵吽字成阿閦佛为成身会者,是三部中之金刚部曼荼罗,即浅略曼荼罗也。又瑜只经序品所说之曼荼罗,是三部中之佛部曼荼罗,秘密曼荼罗也。故瑜只经非十八会之摄,经自说谓为本有金刚界。大日如来住于众生本有之三摩地,于本有无作之境界所现之自性所成三十七尊,故其种子三形等大与常途之说相相异。即浅略者乃加持修生之曼荼罗,秘密者乃本有本地之曼荼罗,胎藏本地之理佛更住于西方莲华部加持现曼荼罗,又本有金刚之智佛更住于东方金刚部示修生曼荼罗,故深秘之两部于佛部中建立之,浅略之两部于莲金二部为其体也。见秘藏记本,同钞一。
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