Objective: On the basis of the random mutagenesis library of ARS, we wish to preparation by screening new ARS sequence which not only have higher ARS activity, but also display higher transformation frequency, in order to constructed new gene recombination vector which have higher efficiency in the Nonconventional yeast expression system.

We have established TCS random mutant library, and therefore, it facilitates theprokary.

Many research groups successfully rely on whole-gene random mutagenesis and recombination approaches for the directed evolution of enzymes.

In vitro molecular directed evolution of enzymeis a new strategy in the engineering of novel biocatalysts, which mainly mimics the process of natural evolution to generaterandom mutation in the genes coding for useful enzymes by the techniques of error-prone PCR, and then in vitro recombinespositive mutations through various methods such .

Several receptor binding sites of LT were mutated randomly, then the R46,S106,L130 combined site-directed random mutation library and S106～F110 region random mutation library were displayed on a filamentous phage surface.

To construct the combined site_directed random mutation library of recombinant human Lymphotoxin (rhLT) for in vitro molecular evolution study, and to study the structure and function relationship.

Generation of a rice mutant library by shotgun antisense gene silencing and mutant screening;

The mutant library/population of Brassica napus including many .

In this review, we describe the types of gene trapping mediated by T-DNA, the advances in gene cloning, and the mutant pool and its applications.