we isolated and microdissected the 24 rice chromosomes and 5 fragments from chromosome I by improved microdissection apparatus, not only resolving the identification and microdissection of rice chromosome, but also reducing the microdissected fragment to 7Mb range, with SUP PCR (single unique primer PCR) that can obtain μg class from fg elass DNA templates.

The single chromosome of Cunninghamia lanceolata was successfully dissected by "glass needles" under the micromanipulator in this research.

Single chromosomes of pomelo ( C.