1) genome walking library
基因组步行文库
2) Genomic library
基因组文库
1.
Construction and screening of genomic library from Raji cells;
Raji细胞基因组文库的构建与筛选
2.
Construction of a bacterial artificial chromosome (BAC) genomic library of Heilongjiang River carps (C. carpio haematopterus)
黑龙江野鲤细菌人工染色体基因组文库构建
3.
DNA of GXP04 was digested with EcoRⅠ,and ligated with cosmid vector pLAFR3 to construct a genomic library.
提取琼氏不动杆菌GXP 04的总DNA,采用限制性内切酶E coRⅠ酶切处理后,构建以pLAFR 3为载体的基因组文库。
3) genomic DNA library
基因组文库
1.
Using the pSVhygB the genomic DNA library of Magnaporthe grisea was constructed.
用潮霉素B(hygromycinB)抗性基因和λ噬菌体的cos位点组建了一种cosmid质粒pSVhygB,以此质粒建立了稻瘟病菌的转化体系,并且构建了病菌的基因组文库。
2.
Results:A genomic DNA library was constructed which included 11520 clones with average insert sizes about 1.
目的:建立贵阳腐霉(Pythium guiyangense Su)基因组文库以便克隆该真菌的基因特别是与灭蚊毒力有关的基因。
4) genome library
基因组文库
1.
There are many ways to achieve the purpose,such as saving living bodies,establishing stem cell bank,mutant bank,generative cell bank,cDNA library,genome library and so on.
动物遗传资源保存方法多种多样,如活体保种、干细胞库、突变体库、生殖细胞库、cDNA文库、基因组文库等。
2.
The rat genome library which has PBR322 as vector and 4~10 kb DNA segments cut by BamHI restriction enzyme as aim segments has been successfully constructed and identified.
以质粒PBR322为载体、BamHI部分酶切片段(4~10kb)为目的片段成功构建了大鼠基因组文库并进行了鉴定,文库总重组子数为3。
3.
In order to study the structures and functions of APP surface proteins,the genome library was constructed.
为研究该细菌表面蛋白的结构与功能,选取APP代表菌株,提取其基因组DNA,用TSP5091随机消化成3~8 kb的片段并回收,连接到预先消化的λZAPII载体上,经过包装、扩增和滴定后,成功地构建了APP基因组文库。
5) genomic DNA walking
基因组DNA步行
6) genome-walking PCR
基因组步行PCR
补充资料:基因组文库
分子式:
CAS号:
性质: 用限制性内切酶切割整个基因组DNA,可以得到大量基因组DNA片段,将这些片段与载体连接、转化细菌、形成克隆,这样一个克隆内的每个细胞的载体上都含有特定的基因组片段。这样的一套克隆就叫做基因组克隆,其中克隆的一套基因组片段就叫做基因组文库。
CAS号:
性质: 用限制性内切酶切割整个基因组DNA,可以得到大量基因组DNA片段,将这些片段与载体连接、转化细菌、形成克隆,这样一个克隆内的每个细胞的载体上都含有特定的基因组片段。这样的一套克隆就叫做基因组克隆,其中克隆的一套基因组片段就叫做基因组文库。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条