1) replication-defective recombinant adenovirus
非复制型重组腺病毒
1.
Objective To apply gene therapy to ischemic flap in plastic surgery, we obtained three strains of replication-defective recombinant adenoviruses expressing hFGF2 , hVEGF165 and hVEGF121 respectively.
目的 构建三株含有hFGF2、hVEGF165、hVEGF121基因的非复制型重组腺病毒，以期用于皮瓣血运障碍的基因治疗。
2) Replication deficient recombinant adenovirus
复制缺陷型重组腺病毒
1.
Replication deficient recombinant adenovirus carrying green fluorescent protein (GFP) was used as a model virus to establish a method to analyze the invasiveness of virus aerosols in vital cells.
将带有绿色荧光蛋白的复制缺陷型重组腺病毒,作为模拟病毒建立一种检测病毒侵袭力的方法。
2.
Objective:To construct 2 kinds of replication deficient recombinant adenoviruses in bacteria rapidly:AdHGF inserted with human hepatocyte growth factor (HGF) cDNA;AduPA inserted with non secreted human urokinase type plasminogen activator (uPA) cDNA.
方法 :分别将人 HGF和 u PA c DNA片段与穿梭载体 p Track- CMV连接 ,线性化后与骨架载体 Ad Easy- 1在大肠杆菌 BJ5 183内同源重组获得腺病毒载体 p Ad HGF和 p Adu PA,经 2 93细胞包装后得到复制缺陷型重组腺病毒 Ad HGF和 Adu PA;将 Ad HGF和 Adu PA分别体外感染肝细胞株 L0 2 ,以 Northern印迹法和 Western印迹法检测两种病毒在肝细胞中的表达。
5) replication-defective recombinant adinovirus
非复制型腺病毒
1.
Objective For further use in gene therapy of bone tissue engineering, we construct replication-defective recombinant adinovirus including the target gene fragment hBMP-4.
目的 构建含有人骨形态发生蛋白-4基因的非复制型腺病毒表达载体(?)AdE／hBMP-4并检测目的基因的表达，以期用于骨组织工程基因治疗的研究。
6) non?replicating vaccinia recombinant virus
非复制型重组痘苗病毒
1.
The non?replicating vaccinia recombinant virus VMAΔCK and the replicating virus VMA which all expressing the major envelope antigen(MA) GP350/220 of EBV were inoculated in the brains of the newborn mice to evaluate the virulence of them.
利用表达EBVGP35 0 / 2 2 0的非复制型重组痘苗病毒VMAΔCK及复制性重组痘苗病毒VMA ,注射乳鼠脑内 ,观察毒力。
补充资料：非甲非乙型病毒性肝炎
非甲非乙型病毒性肝炎
病名。病毒性肝炎类型之一。详见该条。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条