1) 3ˊRACE
3ˊRACE
2) 3'RACE
3'RACE
1.
Identification of differential expression gene on C.Albicans phenotype using 3′RACE after LongSAGE library construction;
运用3′RACE验证LongSAGE文库构建后白念珠菌不同菌相的差异表达基因
2.
The authentic 3′ terminal sequences of genomes of duck hepatitis virus(DHV) serotype Ⅰ strain C80 and serotype Ⅰvariant strain E63 were obtained by using 3′RACE and RT-PCR techniques.
用3′RACE和RT-PCR扩增并克隆鸭肝炎病毒(Duck hepatitis virus,DHV)Ⅰ型毒株C80和Ⅰ型变异株E63的3′末端序列。
3.
With this special primer as up primer and 3′sites Adaptor Primer as down primer,3′-cDNA end of oglc13Ⅱ was rapid amplified by following the instruction of 3′RACE kit(Takara).
01%的HgCl2诱导处理24h之后的燕麦(Avena Sativa)幼叶作为材料,利用RT-PCR技术得到Oglc13Ⅱ的部分cDNA片段,根据此片段设计一条特异性上游引物,反转录引物中的部分序列即3′sites Adaptor Primer作为下游引物,按3′RACE试剂盒(Takara)操作流程进行Oglc13Ⅱ3′末端cDNA的快速扩增,成功获取837bp的3′-RACE反应产物,通过BLAST技术,发现该片段与许多植物β-1,3-葡聚糖酶基因具有较高的相似性(50。
3) 3 RACE
3'RACE
1.
3 RACE Amplification and Sequence Analysis of a Strain of Norovirus;
一株诺如病毒的3’RACE扩增及基因序列分析
2.
Objective:To amplify the 3 -cDNA end of phospholipase D(PLD)gene of Agropyron mongolicum Keng by 3 RACE technique and to analyze the sequence in the experiment,the 3 -cDNA end cloning establish foundation in order to obtain the cDNA sequence and research the function of PLD gene.
目的:采用3’RACE方法对蒙古冰草磷脂酶D(PLD)基因3’端全序列进行了快速扩增和序列分析,为得到全序列及研究该基因的功能奠定基础。
3.
The cDNA of DMrt1(DM-related transcription factor1)was amplified by 3 RACE-PCR(Rapid Amplification of cDNA Ends-Polymerase chain reaction)strategy using the total isolated RNA as template.
从奥利亚罗非鱼精巢提取总RNA,采用RT和3’RACE(rapid amplification of cDNA ends)法分离和测定了奥利亚罗非鱼DMRT1(DM-related transcription factor1)cDNA的3’序列,扩增得到1086bp的片段,阅读框共编码254个氨基酸,序列分析结果表明,奥利亚罗非鱼DMRT1基因的氨基酸序列与尼罗罗非鱼DMRT1基因的同源性为99%,与黑鲷、黄鳝、新月鱼、虹鳟、青鳉鱼等动物的DMRT1cDNA和尼罗罗非鱼、奥利亚罗非鱼等动物的DMO(DM-domain gene in Ovary)cDNA的同源性分别为:80%、78%、73%、62%、41%、26%、26%。
4) 3'-RACE
3-'RACE
1.
In this study,a cDNA fragment of dehydroascorbate reductase(DHAR) including whole 3 -end was cloned from Royal Gala by RT-(nest) PCR and 3′-RACE.
以皇家嘎拉苹果叶片为材料提取总RNA,合成cDNA第一链后,先利用巢式PCR获得苹果脱氢抗坏血酸还原酶(DHAR)基因中间片段,再利用3-′RACE方法进一步获得3′末端核酸序列,共得到840 bp包含完整3′末端的DHAR基因cDNA片段,其序列与烟草、水稻、番茄中DHAR基因序列的同源性均大于70%,证明所克隆到的核苷酸片段确为苹果DHAR基因cDNA片段。
5) 3 RACE
3 RACE
6) 3-RACE
3'-RACE
1.
Amplification Carbo-tip of L Gene by 3 -RACE and Sequence Analysis of Rabies Virus;
RV野毒株L基因C端3’-RACE扩增及序列分析
2.
Methods:An objective gene was isolated by using a RT-PCR and 3 -RACE approachs and two degenerate PCR primers against conserved sequences in the MADS-box and in the C-terminal region of several plants.
方法:根据多种植物的MADS-box保守区及其C-端序列设计一对简并性引物,利用RT-PCR及3’-RACE的方法分离目的基因。
参考词条
补充资料：N,N,Nˊ,Nˊ-tetra-methylethylenediamine
分子式：(CH3)2NCH2CH2·N(CH3)2
CAS号：
性质：无色液体，有强烈胺臭。熔点-55℃。沸点120～122℃。折射率1.4196。能与水、醇和其他有机溶剂相混溶。由甲酸、乙二胺及甲醛回流制得。是极性非质子溶剂。也用作制备烯基炔化物等的有机合成试剂。
CAS号：
性质：无色液体，有强烈胺臭。熔点-55℃。沸点120～122℃。折射率1.4196。能与水、醇和其他有机溶剂相混溶。由甲酸、乙二胺及甲醛回流制得。是极性非质子溶剂。也用作制备烯基炔化物等的有机合成试剂。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。