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1)  content of nitrogen
N%
2)  n (N)/ n (P)
n(N)/n(P)
3)  n(SiO 2)/n(F)
n(SiO2)/n(F)
4)  N,N'-bis(2'
N,N'二2'
5)  n(SiO 2)/n(Al 2O 3)ratio
n(SiO_2)/n(Al_2O_3)
6)  n-6/n-3 polyunsaturated fatty acid ratio
n-6/n-3PUFA
1.
Methods After MCF-7(ER + )and MDA-MB-231(ER - )cells were treated respectively with n-6/n-3 polyunsaturated fatty acid ratio (1-10), Ki-67 expression in nuclear by immunocytochemistry, connexin 26 and 43 expression in cytoplasm and membrane by Western blotting, and GJIC by scrape-loading dye transfer method were performed.
方法N-6/N-3PUFA不同比例(1~10)处理MCF-7(ER+)和MDA-MB-231(ER-)乳腺癌细胞,采用免疫细胞化学观察细胞核KI-67表达,WESTERN BLOT检测细胞膜和细胞质缝隙连接蛋白CX26、CX43表达,划痕负载染料迁移法分析细胞缝隙连接通讯功能。
2.
Effects of different n-6/n-3 polyunsaturated fatty acid ratio on insulin-like growth factor receptor-1 expression in human breast cancer cells;
方法用不同比例的n-6/n-3PUFA(1~10)处理细胞,MTT法检测细胞增殖能力变化;RT-PCR检测细胞IGF-1R mRNA表达变化;Western blot检测IGF-1R蛋白在细胞膜的表达变化。
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性质:又称(-)-α-甲基苄胺[(-)-α-methyl-    benzylamine]。沸点187℃;折射率1.5260;相对密度0.940;旋光度-39°(纯)。具腐蚀性。有毒。用苯乙酮与甲酸铵为原料,经洛伊卡特反应(Leuckart reaction),先制得外消旋体,再用右旋酒石酸拆分制取。在有机合成中用作手性中间体及外消旋羧酸的拆分试剂。

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