1) BMSCs of GFP transgenic mouse
GFP转基因小鼠BMSCs
1.
Objective: To construct the eukaryotic expression vector of human nerve growth factor beta by applying β-NGF gene and to explore the new method of cultivating and purifying BMSCs of GFP transgenic mice for further observing the expression pcDNA_3-β-NGF in BMSCs of GFP transgenic mouse .
目的:构建人β-神经生长因子基因(β-NGF)的真核细胞表达载体pcDNA_3-β-NGF,探讨绿色荧光蛋白(Green flurescent protein,GFP)转基因小鼠骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)体外培养和纯化方法,进一步观察人pcDNA_3-β-NGF重组载体在GFP转基因小鼠BMSCs中的表达。
2) GAD67-GFP transgenic mice
GAD67-GFP转基因小鼠
3) GFP transgenic mice
GFP转基因小鼠
1.
Methods Bone marrow cells isolated from GFP transgenic mice are directly planted in culture flask and an exchange of the total volume medium is made at different time.
方法分离GFP转基因小鼠骨髓细胞直接种植在培养瓶中,分别于2h、4h、6h、8h、10h、12h、14h、16h、24h和48h进行首次全量换液,培养3d后按细胞类型对贴壁细胞分别计数,并用CD44、CD45、CD54进行免疫细胞化学染色。
6) GAD67-GFP knock-in mouse
GAD67-GFP基因敲入小鼠
1.
Association of 5-HT-like and substance P-like immunoreactive terminals with mesencephalic trigeminal nucleus neurons in the GAD67-GFP knock-in mouse;
GAD67-GFP基因敲入小鼠5-羟色胺样和P物质样阳性终末与三叉神经中脑核神经元的联系
2.
Colocalization of KCC2 or NKCC1 with GABA in the neurons within the substantia nigra pars reticulata of the GAD67-GFP knock-in mouse
GAD67-GFP基因敲入小鼠黑质网状部神经元GABA与氯离子转运体KCC2和NKCC1的共存
补充资料：世间转法转智转无尽
【世间转法转智转无尽】
世间转者，谓展转摄前众生界、世界、虚空界也。法转者，谓展转摄前法界、涅槃界、佛出现界也。智转者，谓展转摄前如来智界，心所缘佛智所入境界也。盖此三转，皆言无尽者，以世法智之三种，展转含摄，无有穷尽，是名世间转、法转、智转无尽。
世间转者，谓展转摄前众生界、世界、虚空界也。法转者，谓展转摄前法界、涅槃界、佛出现界也。智转者，谓展转摄前如来智界，心所缘佛智所入境界也。盖此三转，皆言无尽者，以世法智之三种，展转含摄，无有穷尽，是名世间转、法转、智转无尽。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条