Changes in diurnal rhythms of free cortisol secretion during different phases of menstrual cycle;

The free cortisol in marine fish serum was extracted with chloroform,dried under nitrogen gas,solved in 1∶3 methanol/H2O(V/V) and analyzed by ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry(UPLC-Q-TOF-MS),with dexamethasone as internal standard.

Objective To develop a new method for measurement of urinary free cortisol with the IMMULITE Automated Chemiluminisent System.

To evaluate the significance of plasma ACTH (pACTH), serum cortisol (SC) and urinary free cortisol UFC as well as low or high dose dexamethasone suppression test in Cushing syndrome, the levels of hormones were evaluated in 136 cases of Cushing syndrome.

Measurement of the Singh index, canal flare index, morphological index of the cortex, cortical index and stem-canal fit wa.

02mm Vernier caliper in the second metacarpal bone center point spot,surveys the palm backbone transverse diameter and the medullary cavity of bones width,The diameter of the combined cortical thickness was obtained by subtracting the value of the marrow cavity from that of the bone transverse diameter,according to Barnett formula computation metacarpal cortical index.