Influence of hydroxyapatite coating on sandblasted and acid-etched treated surface of TA2 titanium on differentiation of bone marrow stromal cells into osteoblasts induced by platelet-rich plasma in vitro;

Effect of platelet-rich plasma and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs;

Effects of platelet-rich plasma on mineral characteristics of bone marrow stromal cells;

AIM:To investigate the effect of platelet-rich plasma(PRP) on the regeneration of rats\' periodontal tissue.

For 24 rabbits of them,one side was filled with both artificial bone graft and platelet-rich plasma(PRP) as the experimental group,while the other side was filled with artificial bone graft without PRP as the control group;the other 4 rabbits were not treated as the non-treated control.

Methods Platelet-rich plasma (PRP) was isolated by two-step centrifugation.

Platelet rich plasma is an autologous concentration of platelet that has a 4 to 5-fold or more increase over baseline platelet number,and it is a concentration of the growth factors proved to be actively secreted by platelets to initiate all wound healing.

The whole course of cryopreservated platelets preparation in order included whole blood collection, centrifugation for fresh platelet rich plasma preparation, addition of dimethyl sulfoxide, and freeze in -80℃ refrigeratory and thaw in 38℃ water bath.