消减杂交法,differentially expressed gene,在线英语词典,英文翻译,专业英语

1) differentially expressed gene

消减杂交法

2) Suppression subtractive hybridization

抑制消减杂交法

Suppression subtractive hybridization (SSH) method was employed to analyze the differently expressed cDNA sequence between the strained VSMCs pretreated with and without wortmannin, a specific inhibitor of PI3K.

方法应用FX-4000T细胞应变加载系统,对大鼠主动脉VSMCs施加1Hz、10%张应变,Western blotting检测细胞在不同加载时间下Akt磷酸化水平的变化;用抑制消减杂交法(SSH),筛选给予PI3K的抑制剂Wortmannin和给予DMSO两组细胞在加载张应变24h后的差异表达基因片段,得到的消减杂交产物连接到T载体上,经过蓝白斑筛选,对所有阳性克隆进行菌液PCR筛选及测序,应用BLASTN进行序列比对。

3) improved subtractive hybridization

改良消减杂交法

In order to study the mechanism of hyperplasy and invasiveness of fibroblast\|like synovial cells(FLS)of rheumatoid arthritis(RA)patients,an improved subtractive hybridization was used to screen for the genes which expression was increased in RA FLS compared with FLS of osteoarthritis (OA) patients.

为了研究类风湿性关节炎 (rheumatoid arthritis,RA)滑膜细胞 (fibroblast- like synovialcells,FLS)过度增殖和破坏软骨的分子机理 ,利用改良消减杂交法以骨性关节炎 (osteoarthritis,OA)病人滑膜细胞为对照 ,筛选 RA滑膜细胞中的高表达基因。

4) removel

消减

The result show that the removel rate of total phosphate、total nitrogen、Cr~ 6+、COD、volatile phenol、BOD_5 was 33%、21.

结果表明,植被单体对重金属离子,有机污染物及富营养化TN、TP主要指标均具有消减作用。

5) Risk reduction

风险消减

6) Stress subduction

应力消减

参考词条

抑制消减消减板块振动消减消减措施消减噪声消减系数自然消减维数消减约束消减背景消减俯冲消减维度消减消减极性洋壳消减 N-烷化反应玻璃纤维帘布

补充资料：消减

1.减少;降低标准。 2.谓消瘦。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

首页 \| 翻译 \| 影视 \| 论坛 \| 索引源消减污染消减消减断面消减文库消减杂交消减免疫

1) differentially expressed gene 消减杂交法 2) Suppression subtractive hybridization 抑制消减杂交法 1. Suppression subtractive hybridization (SSH) method was employed to analyze the differently expressed cDNA sequence between the strained VSMCs pretreated with and without wortmannin, a specific inhibitor of PI3K. 方法应用FX-4000T细胞应变加载系统,对大鼠主动脉VSMCs施加1Hz、10%张应变,Western blotting检测细胞在不同加载时间下Akt磷酸化水平的变化;用抑制消减杂交法(SSH),筛选给予PI3K的抑制剂Wortmannin和给予DMSO两组细胞在加载张应变24h后的差异表达基因片段,得到的消减杂交产物连接到T载体上,经过蓝白斑筛选,对所有阳性克隆进行菌液PCR筛选及测序,应用BLASTN进行序列比对。 3) improved subtractive hybridization 改良消减杂交法 1. In order to study the mechanism of hyperplasy and invasiveness of fibroblast\\|like synovial cells(FLS)of rheumatoid arthritis(RA)patients,an improved subtractive hybridization was used to screen for the genes which expression was increased in RA FLS compared with FLS of osteoarthritis (OA) patients. 为了研究类风湿性关节炎 (rheumatoid arthritis,RA)滑膜细胞 (fibroblast- like synovialcells,FLS)过度增殖和破坏软骨的分子机理 ,利用改良消减杂交法以骨性关节炎 (osteoarthritis,OA)病人滑膜细胞为对照 ,筛选 RA滑膜细胞中的高表达基因。 4) removel 消减 1. The result show that the removel rate of total phosphate、total nitrogen、Cr~ 6+、COD、volatile phenol、BOD_5 was 33%、21. 结果表明,植被单体对重金属离子,有机污染物及富营养化TN、TP主要指标均具有消减作用。 5) Risk reduction 风险消减 6) Stress subduction 应力消减参考词条抑制消减消减板块振动消减消减措施消减噪声消减系数自然消减维数消减约束消减背景消减俯冲消减维度消减消减极性洋壳消减 N-烷化反应玻璃纤维帘布补充资料：消减 1.减少;降低标准。 2.谓消瘦。说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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