1)  antibody affinity chromatography
抗体亲和层析柱
2)  antibody
抗体
1.
Preparation and property of antibody against environmental hormone dimethyl phthalate;
兔抗邻苯二甲酸二甲酯的多克隆抗体制备及特性分析
2.
Preparation of complete anigen and antibody for 2,4-dichlorophenoxyacetic acid.;
2,4-二氯苯氧乙酸完全抗原和抗体的制备
3.
Preparation of pesticide antibody and enzyme labeled antigen synthesis for permethrin;
氯菊酯农药抗体制备及酶标抗原合成
3)  Antibodies
抗体
1.
Preparation of monoclonal antibodies against tetrodotoxin and its anti-tetrodotoxin poisoning effect;
抗河豚毒素单克隆抗体的制备及其抗毒效应
2.
Production preparation of human bFGF monoclonal antibodies and their antitumor activities with MabF7 on melanoma cells B16 in vitro;
人bFGF单克隆抗体的制备及MabF7对黑色素瘤B16的体外抗瘤效应
3.
Evaluation of results of identification of high virulent Helicobacter pylori strains by detection of serum CagA Antibodies;
CagA抗体检测用于鉴定幽门螺杆菌高毒株感染的评价
4)  antigen
抗体
1.
Isolation and Identification of Duck Hepatitis Virus and Development of ELISA for Detecting DHV Antigen and Antibody;
鸭肝炎病毒的分离鉴定及ELISA检测抗原、抗体方法的建立
2.
The detection is based on the change in the potential from beginning to end of the antigen antibody reaction.
用戊二醛交联法在铂电极上固定IgG抗体 ,制备了可重复使用的电位型无标记IgG免疫探针 。
5)  antiserum
抗体
1.
Preparation and application of nerve growth factor antiserum;
神经生长因子抗体的制备及其应用
2.
When chloroplasts were treated with the antiserum against CBP, Hill reaction activity was inhibited and the intensity of the slow phase of millisecond delayed light emission (msDLE) was reduced.
采用BA-Sepharose6B亲和层析法从小麦叶绿体中纯化了CTK结合蛋白并制备了抗体。
6)  Ab
抗体
参考词条
补充资料:亲和层析
分子式:
CAS号:

性质:又称亲和色谱。利用生物大分子所具有的专一亲和力而设计的一种层析技术。用连接在基体的配位体(如酶、抗体、激素等)作为固定相,使与蛋白质或其他大分子发生可逆的高选择性的相互作用,利用不同亲和力进行分离的液相色谱法。(1)酶-底物或底物类似物或抑制剂或辅因子(辅酶、金属离子等);(2)抗体-抗原或病毒或细胞;(3)激素、维生素-受体蛋白或载体蛋白;(4)外源凝集素-多糖化合物或糖蛋白或细胞表面受体蛋白或细胞;(5)核酸-互被碱基链段或组蛋白或核酸聚合酶或核酸结合蛋白。层析柱的制备是将配体共价偶联到一个不溶性的基物上。样品溶液通过此层析柱时,与此配体能特异结合的组分便被吸附而与其他组分分开。然后,在不能发生特异结合的条件下用某些试剂淋洗以将该物质与配体重新拆开,这样便可获得纯化后的该组分。亲和层析法具有简便、快速、高效且分离产物不会失活等优点。其主要用途是分离和提纯蛋白质、膜和多糖类物质。

说明:补充资料仅用于学习参考,请勿用于其它任何用途。