1) site-direct mutagenesis
定点回复突变
2) Reverse mutation
回复突变
1.
Results:The extract could induce the reverse mutation of E.
coliND 16 0菌株回复突变 ;②可明显诱导大鼠肝原代细胞UDS((P <0 。
4) site-directed mutagenesis
定点突变
1.
Site-directed mutagenesis of functional gene fragment producing CTX-M-14-type extended-spectrum β-lactamase;
CTX-M-14型超广谱β-内酰胺酶基因的定点突变研究
2.
Study on site-directed mutagenesis and kinetics of trehalose synthase from Thermobifida fusca;
Thermobifida fusca海藻糖合成酶的定点突变及其动力学性质研究
3.
The Expression of hBmp-4 in the Pichia Pastoris and Its Site-Directed Mutagenesis;
hBmp-4在甲醇酵母中的表达及其定点突变
5) Site directed mutagenesis
定点突变
1.
Using recombinant glucagon expression system,the 21st aspartic acid was changed to be alanine by means of site directed mutagenesis.
在获得重组胰高血糖素基因工程菌基础上 ,利用定点突变技术改造其第 2 1位氨基酸天冬氨酸为丙氨酸 ,并经DNA测序证明胰高血糖素基因发生了点突变 。
2.
Two p 16 mutants, P48L and D74N, were obtained by site directed mutagenesis using two step PCR method.
应用ＰＣＲ体外定点突变技术，构建了ｐ１６－Ｐ４８Ｌ和ｐ１６－Ｄ７４Ｎ突变体。
3.
Human brain derived neurotrophic factor gene was used as template for oligonucleotide based site directed mutagenesis and PCR.
应用寡核苷酸诱导的定点突变和ＰＣＲ技术对人脑源性神经营养因子基因进行突变，并完成了测序鉴定。
6) site-directed mutation
定点突变
1.
A site-directed mutation at 2753 bp of the ret cDNA induced by PCR;
PCR介导的ret基因体外定点突变
2.
Four conservative tryptophan sites were selected to actualize site-directed mutation.
选取了四个色氨酸保守性位点,分别进行定点突变,构建了CpeS的四个色氨酸突变体,分别为CpeS(W14I)、CpeS(W69M)、CpeS(W75S)和CpeS(G119C,W140V)。
3.
OBJECTIVE: To obtain sequence of α—glucosidase, SWISS-MODEL Server predicted that enzyme structure, amino acid property and characteristic, to design molecular of alpha-glucosidase,make two site-directed mutation by TaKaRa MutanBEST Kit,express the mutant gene in E.
目的:使用PCR技术获得α—葡萄糖苷酶基因序列,通过SWISS-MODEL服务器对α—葡萄糖苷酶结构进行预测,结合氨基酸的性质特点,对酶蛋白进行分子设计,采用PCR突变试剂盒对其进行定点突变,并在大肠杆菌中表达,测定了重组菌、突变菌的酶活力,为进一步研究α—葡萄糖苷酶结构与功能的关系奠定了基础。
补充资料：回复突变
回复突变
突变基因转变成野生型基因的过程。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条