1) 3-dimention co-culture
三维共培养
1.
Methods: To use ECM Gel as a tissue culture substrate,3-dimention co-culture of the choroids plexus or the Neuro-2A cells with the ventral part of the embryonic spinal motor column(Embryonic 12 days,E12)were made in the substrate.
方法:采用ECM Gel作为组织培养的基质底物,将脉络丛组织或者Neuro-2A细胞株和胚胎12天的腹侧脊髓运动神经柱进行三维共培养,观察脉络丛组织或者Neuro-2A细胞株对胚胎脊髓运动神经元轴突生长的影响。
2.
【Method】: To use ECM Gel (Sigma) as a tissue culture substrate, 3-dimention co-cultures of the choroids plexus of rat or the Neuro-2A cells and the ventral part of the embryonic spinal motor column (Embryonic 12 days) were done in this substrate.
【方法】:采用ECM Gel(Sigma)作为组织培养的基质底物,将大鼠脉络丛组织或者Neuro-2A细胞株和胚胎12天的腹侧脊髓运动神经柱进行三维共培养,观察脉络丛组织或者Neuro-2A细胞株对于胚胎脊髓运动神经元轴突生长的影响。
2) 3D culture
三维培养
1.
To study the proliferation and matrix synthesis of the nucleus pulposus cells in monolayer vs 3D culture system;
平面和三维培养对兔髓核细胞增殖和基质合成影响的实验研究
2.
2D and 3D culture of human dental pulp stem cells;
人牙髓干细胞体外二维和三维培养的实验研究
3) three dimensional culture
三维培养
1.
Construction of a three dimensional culture model for inducing embryonic stem cells differentiation in vitro;
体外构建胚胎干细胞生长分化的三维培养模型
2.
Three dimensional culture model of lung cancer cell line A549 and the study of their cellular behaviors
Matrigel胶的肺腺癌细胞株A549体外三维培养的实验研究
3.
To Study the suitable conditions of the induced differentiation of adipose tissue derived mesenchymal stem cells into chondrocytes in three dimensional culture systems.
在三维培养体系中探索ADMSCs向软骨细胞定向诱导分化的适宜条件。
4) Three-dimensional culture
三维培养
1.
METHODS: Three-dimensional culture syst.
本研究通过细胞三维培养,免疫组织化学方法观察肝细胞癌(hepatocellular carcinoma,HCC)中是否存在血管生成拟态,并探讨其形成过程及结构特点。
2.
Methods The VM emerging was observed in embryonal rhabdomyosarcoma cells (RD) and melanoma cells (B16) by three-dimensional cultures and antibody inhibition experiments, the expression change of CD31, Ang2 and LN-5γ2 in VM formation was detected by immunohistochemistry.
方法应用三维培养技术、抗体阻断技术等观察人胚胎性横纹肌肉瘤细胞株RD细胞及小鼠黑色素瘤细胞株B16细胞在体外培养时拟态血管的形成能力并检测CD31、Ang2及LN-5γ2在VM形成过程中的表达改变。
3.
The objective of this study is to establish a new stable tissue engineering lung fibroblast (LF) three-dimensional culture system.
应用组织工程技术建立新型稳定、可靠的肺成纤维细胞(LF)三维培养模型的方法。
5) three-dimensional cell culture
三维细胞培养
1.
The three-dimensional cell culture (TDCC) can bridge the gap between monolayer cell culture and animal experiment.
三维细胞培养技术模拟体内的微环境,填补了单层细胞培养和动物实验的鸿沟。
2.
In recent years, MCR phenomenon has been studied by using three-dimensional cell culture technology in vitro.
近年来人们利用体外三维细胞培养技术研究多细胞耐受性,发现其机制主要为三维结构相关的耐受性和接触性耐受性,并发现一些逆转或减弱多细胞耐受性的方法,显示了其作为临床肿瘤单独治疗或联合治疗的应用前景。
补充资料：共培养
共培养
按照一般体外授精、胚胎移植的常规，是将2～8分裂球阶段的胚胎转移到子宫腔内；而在自然妊娠过程，卵子和精子在输卵管壶腹部相遇，结合，受精卵在输卵管内分裂约3天后形成桑椹体才下降到子宫腔内。这也可能是体外受精、胚胎移植中成功率不高的原因。所谓共培养指采用人输卵管壶腹部上皮细胞，在HamF10或Earle培养液中培养6～7天形成结合—片的单层。在这样共培养条件下，78%胚胎可达到桑椹体阶段，69%可达到胚泡阶段。也可用输卵管上皮细胞飘流的培养液。另外，也有人应用成熟卵泡的颗粒细胞或子宫内膜细胞进行共培养，这些都在研究中。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条