1) Transformation of human T cell
SP2/O细地转化
2) SP2/0 cells
SP2/0细胞
1.
To study the apoptosis of malignant carcinoma cells induced by Hantaan virus, SP2/0 cells cultured in vitro were infected with Hantaan virus.
为研究汉滩病毒对肿瘤细胞的诱导凋亡作用,以一定量病毒悬液感染体外培养的SP2/0细胞,接种后一定时间将细胞消化甩片行Gimsa染色观察凋亡细胞核的变化,制细胞悬液以流式细胞仪测细胞周期,并用免疫组化的方法检测凋亡分子Fas和FasL的表达。
2.
Objective To construct the eukaryotic expression vector for mpb64-ag85b-esat-6 fusion gene of Mycobacterium bovis and express in SP2/0 cells.
目的构建牛分枝杆菌mpb64-ag85b-esat-6融合基因真核表达载体,并在SP2/0细胞中表达。
3.
Objective To express human/mouse chimeric antibody against human T lymphocyte CD4 in murine myeloma SP2/0 cells.
方法真核表达质粒PAG4622+CD4VL和PAH4604+CD4VH经酶切和序列测定后,采用电穿孔转染技术将二者共转染SP2/0细胞,经组胺醇和霉酚酸联合筛选阳性克隆,通过ELISA、流式细胞术、RT-PCR和DNA测序的方法进行初步鉴定。
3) SP2/0 cell
SP2/0细胞
1.
METHODS: A total of 5×106 SP2/0 cells were inoculated subcutaneously in the left hide legs of 208 F2 mice derived from BALB/c and C57BL/6J strains.
背景与目的:我们的研究发现BALB/c小鼠是SP2/0细胞移植瘤的敏感小鼠,而C57BL/6J小鼠则是抗性小鼠。
2.
Soluble tissue factor(sTF) was successfully expressed and secreted in an active form by transfecting the expression plasmid construct containing sTF gene into mouse SP2/0 cells.
用阳离子聚合物法将构建好的转染质粒pIRESneo3-TF1-218转染入SP2/0细胞,经G418抗性筛选后,蛋白可由SP2/0细胞无血清培养分泌表达。
3.
The SP2/0 cells were treated with crude polysaccharides from mycelium of Cordyceps sinensis in vitro to explore the effect of crude polysaccharides on induction of apoptosis and expression of p53 gene in SP2/0 cells.
观察冬虫夏草菌丝粗多糖对SP2/0细胞凋亡诱导和p53基因表达的影响。
4) mixture of SP~2 pattern
SP2型杂化
6) sp2-sp3 hybridized
sp2-sp3轨道杂化
1.
Comparing the molecular structures,molecular orbits,band structures,and density of states,we analyzed the effect of sp2-sp3 hybridized bonding in diamond-like carbon films on space structures and bonding properties.
以强σ键连接的sp2和sp3杂化碳饱和簇模型,即金刚石和石墨两相和团簇模型为研究对象,通过分子结构、电荷分布、能带结构、电子态密度和分子轨道的第一性原理计算和分析,研究了类金刚石薄膜中sp2-sp3轨道杂化的空间结构稳定性的成键特性等。
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1.神名。
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