2) Pichia pastoris
毕赤氏酵母菌
1.
SpreS1 has successfully expressed intracellular in Pichia pastoris,for the purpose of facilitated purification,6-Histidine-tag and 6-Histidine-EK-tag were separately introduced into 3 and 5 terminus of SpreS1-encoding DNA and the fusion genes were subcloned into the expression vector pPIC3.
5k-SS1-6His转化毕赤氏酵母菌GS115,筛选鉴定得到的重组酵母工程菌进行诱导培养,然后采用镍离子柱(Ni-NTABasin)纯化表达产物,纯化的蛋白样品经ELISA效价测定、SDS-PAGE和WesternBlot分析,结果表明融合抗原基因6His-EK-SS1和SS1-6His在毕赤氏酵母中均能表达,纯化的产物仍有免疫原性,所用的纯化方法简便有效。
2.
The recombinant plasmid was integreted into Pichia pastoris X 33 by electroporation and the expressed products were analyzed with SDS PAGE and Western blotting.
以 pPICZαB为载体 ,应用RT PCR从感染D2V的C6 / 36病变细胞中克隆全长E基因 ,电转化法将重组质粒整合入巴斯德毕赤氏酵母菌 ,经抗生素筛选、表型鉴定和PCR分析得到Mut+ 型的多拷贝整合菌 ,经甲醇诱导培养可产生 6 9kD的融合蛋白 ,与含组氨酸尾的D2V包膜糖蛋白分子量理论值相符 ;免疫印迹证实该表达产物可与D2VE特异性单抗和D2V多抗进行反应 ;表达产物经金属螯合亲和层析可获得纯化的含组氨酸尾的E融合蛋白并保留其免疫反应性。
3) Pichia pastoris
毕氏酵母
1.
Oxygen uptake and transfer in recombinant Pichia pastoris cultivation;
氧在重组毕氏酵母发酵过程中的摄取与传递
2.
Specific Growth Rate Control for Pichia pastoris Fed-Batch Cultivation;
毕氏酵母流加发酵过程的比生长速率控制
3.
Expression of NS5ATP13 transregulated by HCV NS5A in Pichia pastoris;
HCV NS5A反式激活基因NS5ATP13在毕氏酵母中的表达研究
4) Pichia pastoris
毕赤氏酵母
1.
Intracellular Expression of Human Apolipoprotein AⅠGene in Yeast Pichia pastoris;
人载脂蛋白基因apoAⅠ在毕赤氏酵母中的胞内表达
2.
Expression of Human Insulin-like Growth Factor I in Methylotrophic Yeast Pichia pastoris GS115;
人胰岛素样生长因子Ⅰ在毕赤氏酵母中的表达研究
3.
Isolation and Characterization of P_(AOX2) Mutant in Pichia pastoris;
毕赤氏酵母醇氧化酶-2基因启动子突变体的分离和鉴定
5) Pichia pastoris
毕赤酵母菌
1.
Purification of GLP-1/albumin Expressed in Pichia pastoris;
在毕赤酵母菌中表达的hGlp-1-白蛋白融合蛋白的纯化
2.
Secreted Expression of Signaling Protein 14-3-3 of Schistosoma japanicom in Pichia pastoris System with Primary Evaluation on its Antigenicity;
日本血吸虫信号蛋白14-3-3在毕赤酵母菌中的分泌表达及其抗原性分析
3.
Expression of anti-keratin human Fab in Pichia pastoris and optimization of expression condition;
人源性抗角蛋白抗体Fab段在巴氏毕赤酵母菌中的表达及表达条件的优化
6) Pichia stipitis
树干毕赤氏酵母
1.
Pichia stipitis Xylitol dehydrogenase (XDH) Gene XYL2 was amplified by PCR.
通过PCR方法克隆得到树干毕赤氏酵母木糖醇脱氢酶(XDH)基因XYL2。
补充资料：贝克氏酵母菌