1) subtractive screening
消减筛选
1.
METHODS:Whole-cell subtractive screening with phage display 7TM peptide library was performed on HepG2 cells and normal L-02 cells.
方法:以L-02为阴性消减细胞,HepG2为靶细胞,对噬菌体随机七肽库进行4轮消减筛选。
2) subtraction biopanning
差减筛选
1.
Methods Osteosarcoma cells os-732 were used as the target cells and osteoblasts as the absorber cells for subtraction biopanning from a 12-mer peptide phage-display library.
方法以骨肉瘤细胞os-732为靶细胞,成骨细胞为吸附细胞对噬菌体12肽库进行差减筛选,用细胞ELISA、免疫组化鉴定阳性噬菌体克隆并测序。
2.
Methods With the LoVo cells as the target cells and human normal large intestinal mucosal epithelial cells as the absorber cells for subtraction biopanning from a c7c phage-display peptide library, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immuno.
方法以大肠癌LoVo细胞株作为靶细胞,人正常结肠黏膜上皮细胞为吸附细胞对噬菌体展示环七肽库进行差减筛选,采用细胞ELISA与免疫荧光检测鉴定噬菌体阳性克隆并测序。
3) counter-selecting
减差筛选
4) subtract biopanning
减性筛选
5) screening by PCR substration
PCR差减筛选
6) antifoam screening
消泡剂筛选
补充资料：消减
1.减少;降低标准。 2.谓消瘦。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条