Method: Transient cotransfection reporter gene assays were performed with pCI-hPXR-neo,pGL3-CYP3A4-Luc and β-galactosidase expression plasmid in HepG2 cells.

Genetic variants of pregnane X receptor in Chinese healthy Han;

Recently identified nuclear receptors,such as the pregnane X receptor,the constitutive androstane receptor,and the vitamin D-binding receptor,play an important role in the induction of metabolizing enzymes an.

The pregnane X receptor(PXR;NR1I2) is an important component of the body′s adaptive defense against xenobiotics including drugs.

SD (sprague-dawley) rat(Rattus norvegicus) hepatocytes were treated with clofibrate and the express levels of its PXR (pregnane X receptor) and CYP3A1 (cytochrome P450 3A1) were determined by Western blot and RT-PCR.

Single nucleotide polymorphisms of pregnane X receptor gene NR1I2 in Chinese healthy Han;

Genistein,coumestrol and quercetin activate pregnane X receptor-mediated transcription of CYP3A4;

Yeast two-hybrid was applied to screen human breast cDNA library,different domains of human progesterone receptor B isoform(hPRB),as the baits respectively.