1) LNA probe
LNA探针
1.
A novel LNA probe real-time fluorescence RT-PCR assay was used for a high-throughput and rapid screening of Japanese encephalitis virus carried from mosquitoes.
方法:从蚊媒监测点采集蚊标本,研磨后提取RNA,用新型的LNA探针实时荧光RT-PCR方法进行高通量快速筛查蚊体携带的乙型脑炎病毒,对核酸阳性的标本进一步用乙型脑炎病毒E基因特异引物进行RT-PCR扩增和序列测定,根据所测序列与GenBank中不同地理来源乙型脑炎病毒株E基因核苷酸序列分析的结果进行基因型别的判定。
2.
MethodsA LNA probe was designed and synthesized to replace the MGB probe for the established TaqMan RT-PCR assay, and then optimized with a known dengue virus standard strain.
方法设计合成LNA探针,替代MGB探针,建立一步法TaqMan RT-PCR方法,并用已知的登革病毒标准毒株进行方法的优化;用商品化的纯化试剂盒和简单的热释放法获取登革病毒细胞培养液和模拟含登革病毒血清中RNA,以优化的TaqMan RT-PCR方法进行检测,比较有或无RNA纯化步骤检测的敏感性和重复性。
2) Probe
[英][prəʊb] [美][prob]
探针
1.
Research Development of Spectroscopic Probes of Protein;
蛋白质光谱探针的研究进展
2.
Comparison of linewidth measurement using different probe based on the least square fitting model;
基于最小二乘拟合的不同探针测量线宽的比较
3.
DNA probe and its application in food industry;
DNA探针及其在食品工业中的应用
3) tip
[英][tɪp] [美][tɪp]
探针
1.
The Measurement of the Applied Foree of a STM Tip to a Measured Surface;
扫描隧道显微镜探针作用力的测量
2.
The tip's radius of curvature and the relative position to the surface of the sample that influenced the distribution of the surface electric field,thus affected the structure of the oxide.
利用原子力显微镜AFM进行阳极氧化纳米刻蚀加工的实质是电场作用下发生下的电化学反应,探针是影响氧化加工重要的因素。
3.
The imaging mode and the development of tip technologies of atomic force microscopy (AFM) are briefly surveyed.
评述了原子力显微镜(atomic force microscopy,AFM)的成镜模式与探针技术的发展以及在脱氧核糖核酸、蛋白质和多糖等生物大分子结构研究中的应用进展,并展望了原子力显微镜在此领域的发展前
4) Probes
[英][prəub] [美][prob]
探针
1.
Study on the Relationships between Chemiluminescent Properties and Molecular Structures of Some Biacridine Probes;
6种不同取代基的双吖啶类探针的化学发光性质及其构效关系的研究
2.
Methods 60-met Oligo probes were designed according to the conserved region of different genotypes of HCV after BLAST.
方法分别对BLAST检索所得的HCV 4个亚型型特异序列逐一进行分析,设计长度均一的60mer Oligo探针,用芯片点样仪将设计好的探针打印到玻片上制备成基因芯片。
3.
To prepare the microarray probes of Bacillus anthracis,pX01 and pX02 of B.
为制备炭疽芽胞杆菌的基因芯片探针文库,以炭疽芽胞杆菌毒素质粒pX01和荚膜质粒pX02为原材料,用Sau3AI酶切pX01和pX02质粒DNA,TaqDNA聚合酶72℃补平加A,经AT克隆,PCR初步鉴定筛选出炭疽质粒片段的阳性克隆。
补充资料:电子探针(见电子探针显微分析)
电子探针(见电子探针显微分析)
electron probe
气二J一,不f丁electron orobom由不,‘.,*二
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条