1) attachment blook culture
组织贴块培养法
2) method of enzymatic digestion
组织块培养法
1.
In this experi-ment the method of enzymatic digestion and the method of tissue block were combined to prepare the feeder layer of MEFthat on a certain degree Solved this problem.
本实验通过将组织块培养法和酶消化培养法结合起来制备小鼠胎儿成纤维细胞饲养层,在一定程度上解决了这一问题。
4) explant culture
组织块培养
1.
The method of explant culture was used successfully in this study to establish neonate goat intestinal epithelial cell(IEC)line.
本试验利用组织块培养法成功的建立了山羊小肠上皮细胞株。
2.
To establish the method on the primary culture of porcine intestinal epithelial cell,different digestion protocols and explant culture were used to identify the best method.
分别采用酶消化法和组织块培养法,建立了猪小肠黏膜上皮细胞原代培养的方法。
5) Attachment block-culture
贴块培养
6) Muscle explants culture
肌肉组织块培养
补充资料:皮肤睾丸(卵睾)组织和中期羊水细胞培养
皮肤睾丸(卵睾)组织和中期羊水细胞培养
诊法。染色体检测的细胞培养技术之一。取小块皮肤或性腺,用无菌之含双抗的Ha~nks液清洗数遍后,在无菌条件下将标本剪成05~1mm3小块种入25ml小培养瓶内,倒置,加15~2ml培养液(RPMI1640或TC199等配制的培基,37℃,CO2孵箱内静置培养,24小时后翻瓶,使培养物全部浸泡于培养液内,一周不必翻动。此后每3~5天或视培养基pH值下降即液体转黄时换培养液一次,使培养液保持一定pH值,清澈透亮。一般7~10天培养物即开始生长。待细胞长满后可传代,部分细胞可液氮内保存备用;部分细胞以胰酶消化后加秋水仙素,制作染色体标本及显带分析。羊水细胞培养后染色体标本制备与上类同。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条