pSEAP2-control and pAB-Sig-AP-SV40 were transfected into chick embryo cells and MCF-7 cells.

The donors we used were blastodermal cells which carry enhanced green fluorescent protein (EGFP) gene.

Primordial germ cells (PGCs) and blastodermal cells were employed as intermediates to produce transgenic poultry in this report, ﹐bservations of the primordial germ cells in blood samples from duck and transfection in vivo: Primordial germ cells from the circulating blood of duck embryo at stage 14-22 were observed by histochemical techniques in smear preparation.

Two strains of identified as avian nephropathogenic infectious bronchitis virus (NIBV) produced cytopathic effect(CPE)after 7 passages in chicken embryo kidney cell ,and showed the syncytial type of CPE in which the infected cells coalesced with loss of cell boundaries and revealed multinucleated giant cells.

Studies in vitro with chicken embryo kidney cell (CEKC) cultures and tracheal orgsn cultures (TOC)were attempted to assess the pathogenicity of six strains of infectious bronchitis viruses (IBV) (M41, Connecticut, T,Gray, GIBV and NIBV).

The results of neutral red staining and acridine orange staining show that the vacuoles are acid,γ-butyrolactones derivative can suppress the differentiation of chick blastodisc cells to vascular endothelial cells by inhibiting the acid vacuoles in cells.