1) small-disturbance method
小干扰法
2) Small interfering RNA
小干扰RNA
1.
Construction of magnetic small interfering RNA nanoparticles and effects of its transfection on silencing survivin gene expression and inducing human bladder cancer cell apoptosis;
磁性小干扰RNA纳米微粒的制备及其转染人膀胱癌细胞对沉默survivin基因表达影响的研究(英文)
2.
Suppression of vascular endothelial growth factor expression by vector-based small interfering RNA in human tongue squamous carcinoma cell line Tca8113;
载体携带小干扰RNA抑制Tca8113细胞血管内皮生长因子的表达
3.
Targeting the 5 -Non-translated Region of Hepatitis C Virus by Intracellularly Expressed Small Interfering RNA;
细胞内表达的小干扰RNA靶向丙肝病毒5’保守区的研究
3) small interfering RNA(siRNA)
小干扰RNA
1.
Objective: To investigate the inhibitory effect of small interfering RNA(siRNA),targeting human papillomavirus(HPV) 18 E6 gene,on expression of E6,E7 gene of HPV 18 and on P53 protein expression in cervical cancer Hela cells.
目的:以人乳头瘤病毒(human papillomavirus,HPV)18型E6基因为靶点,研究小干扰RNA(small interference RNA,siRNA)对宫颈癌Hela细胞株HPV18基因组中恶性转化基因E6、E7的抑制作用及对细胞内P53蛋白表达的影响。
2.
Objectives:To investigate the expressions of matrix metalloproteinase(MMP) -2,-9 and tissue inhibitors of metalloproteinase (TIMP) -1,-2 in human tongue squamous cell carcinoma(Tca8113) in virto and in vivo,after suppressed the expression of vascular endothelial growth factor(VEGF) by vector-based small interfering RNA(siRNA).
目的:利用小干扰RNA(small interfering RNA,siRNA)抑制人舌癌Tca8113细胞血管内皮生长因子(vascular endothelialgrowth factor,VEGF)表达,观察其对培养细胞和移植瘤基质金属蛋白酶(matrix metalloproteinases,MMPs)-2,-9及基质金属蛋白酶抑制剂(tissue inhibitors of metalloproteinases,TIMPs)-1,-2蛋白表达的影响。
3.
In this study, specific small interfering RNA(siRNA) expressing vectors of ATGL gene was constructed.
1-CMVneo干扰载体中,构建猪ATGL基因的小干扰RNA(siRNA)真核表达载体,并对其进行PCR、双酶切及测序鉴定。
4) Small interference RNA
小干扰RNA
1.
Now there are four main kinds of RNA interference libraries with different construction approaches and molecular form,chemically synthesized small interference RNA(siRNA) libraries,chemically synthesized small hairpin RNA(shRNA) libraries,enzymatically prepared siRNA libraries and enzymatically prepared shRNA libraries.
不同种类的RNA干扰库已经被用于基因功能的研究之中,根据其构建方式和分子形式的不同,可将RNA干扰库分为4种类型,即化学合成的小干扰RNA(siRNA)库、化学合成的小发夹RNA(shRNA)表达库、酶切法构建的siRNA库和酶切法构建的shRNA表达库。
2.
Objective:To construct α-fetoprotein(AFP) small interference RNA(siRNA) expression plasmid for the research of the function of AFP and the gene therapy of AFP related tumors with RNA interference(RNAi) technology.
目的:构建特异性抑制甲胎蛋白(AFP)的小干扰RNA(siRNA)表达载体,为进一步研究AFP基因功能及AFP相关肿瘤的基因治疗奠定基础。
3.
Methods Small interference RNAs(siRNAs) targeting IGF-1 gene were synthesized chemically in vitro.
方法体外化学合成靶向IGF-1基因的小干扰RNA(siRNA),采用脂质体法将siRNA以不同浓度梯度转染C_6细胞株,设非特异的siRNA阳性对照组和未转染siRNA的阴性对照组；同时使用绿色荧光素标记的siRNA异硫氰酸荧光素(FITC)-siRNA转染细胞,于荧光显微镜下观察siRNA转染效率；逆转录聚合酶链反应(RT-PCR)半定量检测siRNA对IGF-1基因表达的抑制作用；流式细胞术检测siRNA诱导细胞凋亡作用。
5) siRNA
小干扰RNA
1.
Construction of an expression vector for high expression of siRNA;
一种高效表达小干扰RNA载体的构建研究
2.
Construction of eukaryotic express plasmid of matrix metalloproteinase inhibitor-1 pRNAT-U6.2 siRNA of rat;
大鼠基质金属蛋白酶抑制因子-1 pRNAT-U6.2小干扰RNA表达载体的构建
3.
Inhibitory effect of siRNA on ICAM-1 expression in liver cancer BEL7402 cells;
小干扰RNA抑制肝癌细胞株BEL7402 ICAM-1基因表达的研究
6) small interfering
小干扰
1.
Construction and identification of Cdh1 small interfering RNA eukaryotic vector;
Cdh1小干扰RNA载体的构建及鉴定
2.
Methods:Several pairs of small interfering RNA (siRNA) for WT1 gene was designed and transfected into MCF-7 cells.
方法:设计制备多对针对WT1基因的小干扰RNA(small interfering RNA,siRNA),转染MCF-7细胞,采用实时定量PCR(real-time quantitative polymerase chain reaction,RQ-PCR)法测定WT1 mRNA的表达,westernblot测定WT1蛋白的表达,流式细胞仪检测加入长春新碱后的MCF-7细胞凋亡率。
3.
Methods:MDR1-targeted small interfering RNA(si-MDR1) was selectively synthesized.
方法:选择合成封闭mdr-1基因的小干扰序列(si-MDR1),以1个碱基突变的si-MDR1-mut为对照序列,在脂质体介导下转染至K562/AO2细胞系。
补充资料：摆放式干扰机(见通信干扰设备)
摆放式干扰机(见通信干扰设备)
hand-emplaced jammer
baifongshi gQnraoii摆放式干扰机(hand一emplaeed jam;ner)见通信干扰设备。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条