Optimization of alkyl-polyglycoside synthesis catalyzed by immobilized enzyme in microcapsules

Combined with the monolithic column and enzyme immobilization technique,an immobilized enzyme micro-reactor for determination of VC was constructed by using a self-made polystyrene monolithic column.

Application of immobilized enzyme in food industry;

Recent progress of carrier materials used for immobilized enzyme by plasma optimization and modification technology;

Approximate solutions for diffusion-reaction problem of immobilized enzyme;

Research status and application on matrices for immobilized enzymes;

Using carrier materials treated by plasma technology exploits a new land of immobilized enzymes.

The development and the mechanism of stereochemical resolution of racemates, in HPLC, using a chiral stationary phase based upon immobilized enzymes,was reviewed with 22 references.

Application of natural polysaccharide on materials used as enzyme immobilization carriers;

Research advances in enzyme immobilization technology and the carrier m aterials;

In order to improve the catalytic properties of enzymes,the author and his group have devoted themselves to the researches of enzymatic molecular modification,enzyme immobilization,non-aqueous enzymatic catalysis and directed enzyme evolution in the past two decades,finding that the catalytic properties of enzymes such as activity and stability.

The surface appearance and condition of the new membrane was analyzed through SEM, after enzyme immobilization and activity testing of the enzyme membrane, the immobilized enzyme based in the new membrane was found that it had quite high catalysis activity and excellent stability.

The aim of the present work is to compare various enzyme immobilizationtechniques used in the design of a pesticide biosensor, which included simple physical adsorption, self assembling mono-layer,sensitization enzyme immobilization technique, bovine serum albumin (BSA)-glutaraldehyde cross-linking method and so on.

Furthermore,the microgels were used as carriers of horseradish peroxidase(HRP),and the conditions and properties of enzyme immobilization had been studied.

Experiments show that nanoparticles can significantly enhance the catalytic activity of the immobilization enzyme.

The function of nanoparticles effect on immobilization enzyme is discussed.

The thesis summarizes the progress of methods, carriers, application of immobilization enzyme, and the design and synthesis of novel reactive carrier materials, also includes study of the optimal conditions of immobilization enzyme and the properties of the immobilized enzyme.