The label-free DNA sensing techniques are currently receiving considerable attention for the de- termination of pathogenic genes and the diagnosis of genetic disease, etc.

The production of marker-free transgenic plants is a critical advance for both plant biotechnology research and commercial development.

In this study, we established a vector-free and marker-free transformation system, the minimum linear exogenous gene was introduced into maize by pollen-tube pathway method, and it mainly solved the biosatety issue of transgenic plants.

Maize immature embryos were co-cultured with Agrobacterium carrying two vectors, one marker-free vector harboring the SCMVirNIb and .