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1)  VHR
人痘苗病毒H1相关磷酸酶
1.
To study the effect of three positively charged arginine residues near the active site Cys 124 of the human dual-specific phosphatase on the catalytic function, six VHR mutants R 125L, R 130L, R 130K, R 130L/S 131A, R 158K and R 158L were obtained using QuikChange site-directed mutagenesis method.
人痘苗病毒H1相关磷酸酶 (VHR)及其突变体进行稳态动力学参数和竞争性抑制常数Ki 的测定 ,结果显示上述Arg130 和Arg158突变体的kcat/Km 值都较野生型有大幅度下降 ,而Ki 值有明显上升 ,表明 130和 15 8位的精氨酸是VHR活性所必需 ,而且可能与底物上带负电的磷酸基团结合有关。
2)  Vaccinia virus
痘苗病毒
1.
Design and preparation of oligonucleotide microarray for vaccinia virus detection;
痘苗病毒寡核苷酸检测芯片的设计及研制
2.
Construction and Identification of Eukaryotic Expression Vector of Vaccinia Virus Expressing Human Interleukin-2 Gene;
重组人白细胞介素-2基因痘苗病毒真核表达载体的构建与鉴定
3.
Coexpression of HIV-1p24gag and hIL-6 gene in recombinant vaccinia virus;
在重组痘苗病毒中共表达HIV-1p24gag与hIL-6基因
3)  T7 vaccinia virus
T7痘苗病毒
4)  GRSPaV
葡萄茎痘相关病毒
5)  recombinant vaccinia virus
重组痘苗病毒
1.
Combination immunization with EIAV Env protein expressed by recombinant baculovirus and recombinant vaccinia virus containing env gene;
重组杆状病毒表达的EIAVEnv蛋白与含有env基因重组痘苗病毒的联合免疫
2.
Expression of human CD20 gene in recombinant vaccinia virus;
利用重组痘苗病毒表达人 CD2 0基因
3.
To construct a recombinant vaccinia virus RVJ1175M2 expressing influenza A3 virus(H3N2) M2 gene,full length gene encoding influenza virus(H3N2) M2 protein was amplified with PCR and cloned into plasmid pJSC1175 which was used for homologous recombination with vaccinia virus Tiantan strain.
为获得表达甲3型流感病毒(H3N2)M2蛋白的重组天坛株痘苗病毒RVJ1175M2,使用PCR方法扩增流感病毒全长M2基因,将其克隆到天坛株痘苗病毒同源重组质粒pJSC1175中,获得重组质粒pJSC1175M2,通过与痘苗病毒载体同源重组,构建了含流感病毒M2基因的重组痘苗病毒株RVJ1175M2。
6)  vaccinia virus Tian Tan strain
痘苗病毒天坛株
1.
To study whether the vaccinia virus Tian Tan strain(VTT) can infect equine cells and produce immune response in horses,recombinant vaccinia virus WR strain expressing green fluorescence protein(GFP) was used to infect fetal dermal donkey cells(FDD) and the virus replication was tested regularly by flow cytometry.
目的研究痘苗病毒天坛株是否能够感染马属动物细胞,并诱导免疫马产生免疫反应。
补充资料:磷酸化酶磷酸酶
分子式:
CAS号:

性质:又称磷酸化酶磷酸酯酶。磷酸酯酶一种,EC3.1.3.17,分子量5×104。它的底物是有活性的磷酸化酶a。它所催化的反应是磷酸化酶a一级结构中14序号上的丝氨酸上与磷酸基相连接酯键水解(断裂)而游离出4个无机磷酸分子,使磷酸化酶a转变为无活性的磷酸化酶b(变构效应物,即AMP可使它成为有活性的酶)。它的作用正好与在ATP(腺苷三磷酸)存在下的磷酸化酶激酶(phosphorylase kinaseEC2.7.1.38.)的作用相反。它在糖原的生成和代谢中起催化和调节的作用。通常磷酸化酶磷酸酶可从兔肌肉组织中提取。

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