1)  Site-directed mutagenesis
寡核苷酸定点突变
1.
Site-directed mutagenesis was performed at Ser177 of Penicillin G Acylase(PGA) gene of E.
本研究用缺口双链法对大肠杆菌青霉素Ｇ酰化酶（ＰＧＡ）基因Ｓｅｒ１７７进行寡核苷酸定点突变。
2)  Oligonucleotides
寡核苷酸
1.
Spectroscopic Study on Sequence Specific Binding of Thionine with Oligonucleotides;
硫堇与寡核苷酸结合序列选择性的光谱研究
2.
Effect of Column Temperature and Flow Rate on Retention and Separation of Oligonucleotides by Reversed Phase Ion Pair Chromatography;
柱温和流速对反相离子对色谱法分析寡核苷酸的影响
3.
Biological effect of c-myc antisense oligonucleotides on MKN-45 cells;
c-myc反义寡核苷酸对胃癌MKN-45细胞株生物学影响的研究
3)  oligodeoxynucleotides
寡核苷酸
1.
Effects of NF-κB “decoy” oligodeoxynucleotides on inducible nitric oxide synthase expression in LPS-induced mouse macrophages;
NF-κB“诱骗”寡核苷酸对小鼠巨噬细胞iNOS表达的影响
2.
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry of oligodeoxynucleotides consisting of isonucleosides with sinapinic acid as matrix was reported.
以芥子酸（sinapinicacid）为基质，对异核苷杂寡核苷酸（3－（R）－IsodT）13·dT和（3－（S）－IsodT）13·dT进行了基质辅助激光解吸电离飞行时间质谱测定，准确地测得分子量，合成的寡核苷酸得以鉴定。
3.
Objective To study the effect of NF-κB decoy oligodeoxynucleotides on IL-8 expression in LPS-induced SW480 cells.
目的探讨核转录因子κB(NF-κB)"诱骗(decoy)"寡核苷酸(ODNs)对结肠癌SW480细胞IL-8表达的影响。
4)  oligonucleotide
寡核苷酸
1.
Triplex-forming oligonucleotide inhibits the expression of tissue factor gene in endothelial cells induced by the blood flow shear stress in rats;
形成三链DNA的寡核苷酸抑制切应力诱导的大鼠内皮细胞组织因子表达
2.
Synthesis of iso-guanosine incorporated G-rich oligonucleotide;
异鸟苷掺入的富鸟嘌呤寡核苷酸的合成
3.
The impact of three fluorescence marker methods to oligonucleotide microarray hybridize signal;
三种荧光标记法对寡核苷酸芯片杂交信号的影响
5)  oligodeoxynucleotide
寡核苷酸
1.
Effects of heparinase antisense oligodeoxynucleotide on cell proliferation and expression of heparinase protein in A375 cells;
乙酰肝素酶反义寡核苷酸对A375细胞增殖及乙酰肝素酶蛋白表达的影响
2.
A Study of Antisense Oligodeoxynucleotides As A Chemo-Sensitizer to Cytotoxic Agents on Proliferation of Breast Cancer Cell Lines in Vitro;
反义硫代寡核苷酸增强细胞毒药物抗乳腺癌活性的体外研究
3.
Inhibitory Effect of Antisense Oligodeoxynucleotides Targeting HER-2 mRNA on Proliferation of Breast Cancer Cell Line in Vitro;
靶向HER-2 mRNA反义硫代寡核苷酸体外抗乳腺癌活性研究
6)  oligonucleotide
寡核普酸
参考词条
补充资料：定点突变法
分子式：
CAS号：

性质：又称定点诱变或定位诱变。采用基因合成技术或取相应的寡核苷酸，用酶法(或化学方法)改变基因中某一点上密码子，再把含有该点突变的基因插入到质粒上，形成重组质粒，经转化把重组质粒引入到酵母菌、大肠杆菌等新宿主细胞中进行表达，其表达的产物是一个突变蛋白或突变酶。采用定点突变的方法，可灵活而巧妙地勾画甚至确定某特定氨基酸残基在蛋白质和酶分子中的功能和作用。也是创建工程化蛋白或酶的工具方法之一。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。