Study on in vitro assay methods for human coagulation factor IX;

To probe the feasibility of efficient production of human clotting factor IX(hFIX)with the approach of mammary gland bioreactor of transgenic animals,we constructed hFIX mammary gland expression vector containing promoter,exon 1,intron 1 and exon 2 of the goat β casein gene about 6.

This study aimed to introduce a site specific point mutation into the human coagulation factor IX gene expressing vectors (pMe4bAIXml plasmid)for microinjection and to obtain transgenic mouse containing copies of a stably integrated pMe4bAIXml plasmid on the basis of coagulation factor IX gene knock out mouse model as an more efficient animal model of hemophilia b.