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1)  Nuclear scaffold-associated region
核骨架结合区
2)  scaffold matrix attachment region
核骨架基质结合区
1.
In order to relieve the retroviral vector mediated gene silencing and further enhance the expression of MFG retroviral vector mediated transgene, the 800 bp scaffold matrix attachment region (S/MAR) from upstream of human β INF gene to the upstream of 3′ LTR of MFG vector in reverse and positive orientation were cloned, respectively.
为减轻逆转录病毒介导的外源基因的沉默 ,进一步提高逆转录病毒MFG载体介导的转移基因的表达 ,将人 β INF基因上游 80 0bp的核骨架基质结合区 (S MAR)分别反向和正向克隆至MFG载体 3′LTR上游 ,以egfp为报告基因观察S MAR对egfp基因表达水平以及对病毒滴度的影响 。
3)  LPTS
细胞核骨架结合元件
4)  scaffold attachment regions(SAR)
核骨架附着区(SAR)
5)  nuclear matrix
核骨架
1.
HeLa cell nuclei were isolated and the nuclear matrix specimens were prepared.
提取HeLa细胞核并制备核骨架标本,以抗肌动蛋白抗体为探针,采用SDS一PAGE、免疫荧光和免疫印迹等方法,对HeLa细胞核和核骨架中的肌动蛋白进行了研究,并用鬼笔环肽荧光染色方法研究了其中的F-肌动蛋白。
2.
(2)Nuclear matrix is involved in DNA replication.
综合分析了国际国内近年来有关核骨架研究的新进展,从几个方面的研究事实,包括核骨架对染色质DNA的有序组织、核骨架参与DNA复制和基因的表达与调控以及核骨架的起源进化等,阐明核骨架是细胞核内染色质结构的有序组织者和功能活动的参与者;核内纷繁复杂的生命活动能有条不紊地进行,核骨架在其中扮演了重要角色。
3.
The results of the immunoblot of anti-β-actin antibody as immunomarker indicated that the cytoplasm,nuclei andnuclear matrix had positive reaction with the antibody.
自烟草悬浮培养细胞中分离出细胞核,提取核总蛋白后,用DNase I消化去除核酸和2MNaCl抽提后制备成细胞核骨架。
6)  matrix attachment region
核基质结合区
1.
Nuclear matrix attachment regions and transgene expression in plants;
核基质结合区与转基因植物的基因表达
2.
Construction of the expression vector which contains two different matrix attachment regions flanking the expression cassette
表达盒两侧不同核基质结合区序列介导表达载体的构建
3.
Objective To clone the human β-globin matrix attachment region(MAR) and construct the mammalian animal expression vector pCAT-MAR, which contains the MAR and CAT reporter gene.
目的克隆人β-珠蛋白核基质结合区(matrix attachment regions,MAR),构建包含MAR及报告基因CAT的哺乳动物载体pCAT-MAR。
补充资料:HeLa细胞核骨架──Lamina体系




HeLa细胞核骨架──Lamina体系  
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  [图]图

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