1) exonuclease Ⅲ
外切核酸酶Ⅲ
1.
Digestion rate markedly increased as exonuclease Ⅲ digesting linear DNA with double chains which had reacted with Bleomycin A 5 Ce(Ⅲ) [BLMA 5 Ce(Ⅲ)].
根据外切核酸酶Ⅲ酶解博莱霉素 Ce (Ⅲ ) [BLMA5 Ce (Ⅲ ) ]作用过的双链直线型DNA时 ,酶解速率明显增大 ,酶解产物除 5′ dAMP、 5′ dGMP、 5′ dCMP和 5′ dTMP 4种单核苷酸外 ,还有其他成分存在的实验事实 ,推测出BLMA5 Ce (Ⅲ )在DNA双链的特定部位沿 5′→ 3′的方向切断磷酸二酯键 ,使DNA的双链上形成多个暴露的 3′ OH末
3) EXON I (Exonuclease I)
核酸外切酶Ⅰ
4) EXON I(exonuclease I)
核酸外切酶I(EXONI)
5) 3' exoribonuclease
3'外切核酸酶
1.
The exosome is a conserved complex which identified in Saccharomyces cerevisiae and has 3′ exoribonuclease activity in vitro.
外切体是酿酒酵母中鉴定的一个保守复合体 ,具有 3′外切核酸酶的活性 。
6) endo-exonuclease
核酸内外切酶
1.
On shifting rapidly growing mycelia culture of Neurospora crassa from 30℃ to 45℃ for 2h,75% of active endo-exonuclease, an enzyme implicated previously in DNA repair, was released from both nuclei and vacuoles into the cytosol where it was inactivated.
将快速生长的粗糙脉孢菌Neurosporacrassa培养液从30℃升温至45℃,热休克处理2小时,75%有活性的核酸内外切酶endo-exonulease(此酶为DNA修复酶)从核及液泡中释放到胞质并以无活性的形式储存起来:用放线菌酮处理,不能阻止此酶的释放,但能使灭活降低;致使胞质的酶活性提高5-15倍。
补充资料:核酸外切酶
核酸外切酶
能将多核苷酸水解以产生单核苷酸的酶(磷酸二酯类)降解作用,从链的一端陆续向另一端进行。可从c-5′端向c-3端(5′→3′核酸外切酶),也可沿相反方向(3′→5′核酸外切酶)进行。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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