说明:双击或选中下面任意单词,将显示该词的音标、读音、翻译等;选中中文或多个词,将显示翻译。
您的位置:首页 -> 词典 -> PCR测序
1)  PCR sequencing
PCR测序
1.
The DNA electrophoretic bands of PCR products were recovered and purified , then sequenced by PCR sequencing method with one of the two primers.
用特异性引物对肌球蛋白轻链 2启动子 (myosinlightchain 2 ,MLC2 ) 糜酶融合基因的转基因新生鼠鼠尾DNA进行PCR筛选 ,低熔点琼脂糖凝胶电泳回收阳性样品PCR所扩出的DNA条带 ,纯化后用同一对引物中的一个进行单引物PCR测序 ,与所转外源基因序列比较 ,进一步确定整合有外源基因的阳性鼠 。
2.
The polymorphisms of POU1F1 gene were analyzed by PCR sequencing technique in two goat populations(Xiao xiang yang,Guizhou Black goat) and the effects of genotypes of POU1F1 gene loci on slaghter traits(naked body weight,dressing percentage,rib eye area,waist muscle thickness, net meat weight,net meat percentage,bone weight,flesh and blood rat) were evaluated.
本研究采用PCR测序技术分析了POU1F1基因内含子4、外显子6在小香羊和贵州黑山羊2个山羊群体中的多态性及其与屠宰性状的相关性,为中国山羊产肉性能的高效选育和山羊品种分子标记数据库的建立提供分子遗传学依据。
3.
Based on the study of physiological-biochemical characters as well as hydrophobic capability and degradation capability,the sequence of 16SrDNA and nutrient balance of hydrophobic hydrocarbon degrading bacteria named HDB-1were analyzed,experimental results showed that PCR sequencing was consistent with cell classification,and hydrophobicities of HDB-1 belong to Pseudomonas.
实验表明,PCR测序与细胞分类法得到的结果一致,菌株HDB-1属于假单胞菌属细菌(Pseudomonas),氮、磷则是影响HDB-1菌降解石油的限制因素。
2)  PCR-sequencing
PCR-测序
3)  PCR-DNA direct sequencing
PCR-DNA 测序
4)  PCR direct sequencing(PCR-DS)
PCR直接测序
5)  cloning sequence analysis
RTPCR序列测定
6)  Sequence analysis with clone of PCR products
PCR产物克隆测序
补充资料:DNA测序
分子式:
CAS号:

性质:确定一段DNA中碱基排列的次序。一般用双脱氧测序法,另外还有一种马克西姆-吉尔伯特法,是依据四个碱基对不同试剂,如哌啶及肼的不同的反应能力实现的。可利用克隆载体上接头的两侧引入已知序列(例如Sp6,T7,T3的启动子序列),当插入目的片段后,即可利用此已知序列的寡核苷酸作引物。常用于未知序列DNA片段的测序。

说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条