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1)  reporter gene
报导基因
1.
Methods: The expression vectors which contained either human muscle specific promoter or SV40 promoter directing the transcription of reporter gene (E.
方法:将表达质粒pM3TnC3、pSV40-LacZ直接注射入Wistar大鼠舌肌中,应用PCR技术、β-半乳糖苷酶(X-gal)组织化学染色及Southern杂交技术分析质粒携带报导基因——乳糖苷酶(LacZ)体内表达情况。
2.
Results:The reporter gene expression was foung in Wistar rat tongue muscle after plasmid DNA injection.
结果:在Wistar大鼠舌肌中检查出报导基因产物β- 半乳糖苷酶的表达。
2)  reporter gene
报告基因
1.
Transfection and expression of retrovirus-mediated reporter genes in retinal pigment epithelial cells in vitro;
逆转录病毒载体携带报告基因在色素上皮细胞的转染与表达
2.
Establishment and its application of a reporter gene-based screening cell model of NF-κB signal transduction to combat Alzheimer s disease;
基于报告基因和NF-κB信号通路的抗阿尔茨海默病药物细胞筛选模型的建立与应用
3.
Monitoring early toxicity of heavy metals including Hg using a HSE-SEAP reporter gene;
报告基因方法监测重金属汞及其化合物的早期毒性
3)  report gene
报告基因
1.
Inducement of ginsenoside Rg_1 on expression of glucocorticoid receptor report gene in HL-7702 cells;
人参皂苷Rg_1诱导人肝HL-7702细胞糖皮质激素受体报告基因表达的作用
2.
Recombinant adeno-associated virus vector-delivered report gene expression in corneal endothelium;
重组腺相关病毒介导的报告基因在角膜内皮细胞中的表达
3.
Construction and identification of human Puma promotor luciferase report gene vector
人Puma启动子荧光素酶报告基因的构建和鉴定
4)  reporter genes
报告基因
1.
Objective To construct bait vector pGBKT7-COPS3 in yeast two-hybrid system GAL4,and explore whether expression product of COPS3 has toxicity to host yeast cells and activates the reporter genes in the yeast two-hybrid system.
目的用COPS3基因片段构建酵母双杂交GAL4系统的诱饵载体pGBKT7-COPS3,并检测其表达产物对酵母细胞有无毒性及对报告基因有无激活作用。
2.
AIM: To construct a bait vector with Bit1 gene in yeast two-hybrid system GAL4, and assay whether Bit1 gene expression product affects the growth of host yeast cells and activates the reporter genes in the yeast two-hybrid system.
目的:用Bit1基因片段构建酵母双杂交诱饵载体,并检测其表达产物对酵母细胞有无毒性作用及对报告基因有无激活作用。
5)  reporter gene
报道基因
1.
With the Green Fluorescent Protein gene (GFP) as a reporter gene, the transgenic embryos, seedlings and calli of cotton(Gossypium hirsutum L.
以绿色荧光蛋白GFP基因为报道基因,用花粉管通道和农杆菌介导的转化方法将外源基因导入棉花(Gossypium hirsutum L。
2.
The endogenous Stat3 protein could also induce the expression of m67 sequence directed reporter genes.
通过免疫细胞化学染色 ,免疫沉淀结合免疫蛋白质印迹法 ,以及瞬时转染由Stat3特异识别DNA元件所指导的报道基因等技术 ,证明COS7细胞中存在活性Stat3蛋白的表达。
6)  Gene transfer
基因转导
1.
Methods The comparative studies in cell viability,GFP gene transfer and its correlated protein expression as well were perfo.
结论BS是一种特异性的细胞收缩抑制剂,能够提高成年小鼠心肌细胞原代培养的细胞成活率并延长培养时间,从而提高由病毒介导的基因转导效率。
2.
With the development of new technologies including gene transfer and tissue engineering,it is possible to reconstruct salivary gland functions.
近年来随着涎腺基因转导及组织工程等新技术方法的应用,涎腺功能重建取得了可喜的进展。
补充资料:报导
报道。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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