The internal ribosome entry site (IRES) from encephalomyocarditis and that from polio viruses were used to construct a polycistronic retroviral vector pGCEN/TRI, where Neoresistant gene was used as a marker gene and TNFα cDNA, IL2 cDNA as genes of interest, so that the LTR promoter derived the expression of a tricistronic mRNA.

Construction of Foxp3 retroviral vector and its suppressive function on the induced CD4~+CD25~+Foxp3~+ T cells;

Construction and expression of recombinant retroviral vectors expressing siRNA for MDR1 gene;

Apoptosis of pancreatic carcinoma mediated by a retroviral vector containing antisense human telomerase gene;

Construction of retrovirus expressing CTGF siRNA and its steady packaging cell line mediated by PRS retrovirus vector;

Construction of recombinant retrovirus vector bearing antisense AFP enhancer/TK gene and its hepatocyte-specific expression;

Report on design and construction of recombinant retrovirus vector expressing siRNA for survivin gene knockdown;

Effect of retrovirus-mediated RNAi on the expression of E2F1 mRNA in breast cancer MCF-7 cell line;

Objective To study the effects of the retrovirus-mediated RNAi technology on suppressing the expressions of E2F1 protein in breast cancer cell MCF-7.

Construction of retrovirus expressing β-scretase substrate-base peptide and its steady packaging cell line mediated by pLXSN retrovirus vector;

Cloning of bovine sox2 gene and construction of its retrovirus vector

This review focused on Haemophilia B s mechanism and the new development of retrovirus vector used in the gene therapy of Haemophilis B.