1) perforated patch
穿孔膜片箝
1.
In order to prevent the “run down” phenomena, amphotericin B in pipette solution was used to make perforated patch , the process of which was described in details.
为防止“衰减”现象的出现(rundown),运用含AmphotericinB的电极液作穿孔膜片箝。
2) Perforated patch clamp
穿孔膜片钳
3) patch clamp
膜片箝
1.
Methods Conventional microelectrode and patch clamp techniques were used to record the PTH-induced changes of action potential and pacemaker current I f in the rabbit sinus node.
方法运用微电极、膜片箝技术,记录PTH对家兔自律性动作电位和兔起搏离子流的影响。
2.
Methods: The delayed rectifier potassium current was recorded by using whole cell arrangement of the patch clamp procedure.
方法 :采用电极内液浓度差扩散法进行细胞内给药 ,利用全细胞膜片箝技术测定单细胞Ik。
3.
Methods Cell attached configuration of patch clamp technique was employed to record the current of BK Ca in ECV304.
方法 用细胞贴附式膜片箝技术记录 BKCa的活动电流。
4) patch-clamp
膜片箝
1.
In the present study, both cell-attached and inside-out patch-clamp techniques wereused to detect the effects of 17β-estradiol (E2) on 42 pS delayed rectifier potassiumchannels in acutely dissociated hippocampal neurons of new-born SD rats.
用膜片箝技术的细胞贴附式和内面向外式,研究17β-雌二醇(E2)对大鼠海马神经元延迟整流型K+通道的影响。
2.
Whole-cell patch-clamp technique was used to study the changes of ionic currents in murine peritoneal exudate macrophages (PEMs ) prestimulated with granulocytemacrophage colony-stimulating factor (GM-CSF, 16 ng/ml) for periods from 0.
5~6d)刺激小鼠腹腔渗出的巨噬细胞,采用全细胞膜片箝技术研究在GM-CSF刺激过程中细胞膜电流的变化,观察到一种CM-CSF诱导的瞬间失活的外向K+电流(记作IA),该电流在生理电压范围内可发生稳态失活,且当施加0。
5) patch clamp
片膜箝
6) perforated-patch clamp
穿孔膜片钳技术
1.
K~+ current of porcine coronary artery SMC recorded by perforated-patch clamp;
穿孔膜片钳技术使电极与细胞胞质之间保持电学连续性,它使细胞内环境保持稳定,利于研究胞内信息传导机制,并可改善常规全细胞膜片钳时破膜所造成的封接稳定性的破坏,有望提高实验的成功率。
补充资料:成都无缝钢管厂管坯在菌式穿孔机上穿孔
成都无缝钢管厂管坯在菌式穿孔机上穿孔
成都无缝钢管厂管坯在菌式穿孔机上穿孔
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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