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1)  longterm culture in vitro
体外长期培养
2)  long-term culture
长期培养
1.
Objective:To investigate the generative or regenerative hair cell and studes of molecular and genetic in the inner ear,the long-term culture systems of utricular sensory epithelial cell of the rats were established.
目的:建立椭圆囊感觉上皮细胞(USEC)长期培养体系,为內耳毛细胞再生研究提供稳定的活性细胞。
3)  culture in vitro
体外培养
1.
Improvement on culture in vitro of rat cerebral cortical astrocytes;
大鼠大脑皮层星形胶质细胞体外培养方法改良
2.
Progress in research on embryo culture in vitro;
胚胎体外培养的研究进展
3.
Mouse cardiomyocytes culture in vitro;
小鼠心肌细胞的体外培养
4)  in vitro
体外培养
1.
Improvement of cultural method in vitro for Echinococcus multilocularis metacestodes and primary identification of culture product;
泡球蚴体外培养方法的改进及培养产物的初步鉴定
2.
The Modulation of Endochondral Ossification in Rat Nasal Chondrocyte Cultures with 45S5 in vitro;
固骼生对体外培养鼠鼻中隔软骨细胞增殖及骨化的初步研究
3.
Effects of DSPP antisense oligodeoxynucleotide on development and mineralization of mouse molars in vitro;
牙本质涎磷蛋白反义核酸对体外培养牙胚发育、矿化的影响
5)  culture [英]['kʌltʃə(r)]  [美]['kʌltʃɚ]
体外培养
1.
Spermatogonial stem cells transplantation and culture:a research progress;
精原干细胞移植及体外培养研究进展
2.
Isolation and Culture of Bovine Fetal Fibroblasts;
牛胎儿成纤维细胞分离与体外培养
3.
Human Bone Marrow Mesenchymal Stem Cell (BM-MSC) Culture and the Expression of Hematopoietic Cytokines;
人骨髓间充质干细胞体外培养及其细胞因子表达的研究
6)  in vitro culture
体外培养
1.
Observation of the protective effects of OCE on retina tissue in vitro cultured;
OCE对体外培养的视网膜组织保护作用的观察
2.
Analysis of influence factors of in vitro fertilization laboratory quality control on in vitro culture of mouse embryos;
IVF实验室质控中影响鼠胚体外培养的因素分析
3.
Preliminary research on in vitro culture of human primordial germ cells;
人原始生殖细胞体外培养的初步研究
补充资料:人体外周血淋巴细胞培养

  
  人体外周血淋巴细胞培养
  

  诊法。为 检查患者染色体最简便的细胞培养技术。先于无菌条件下制备RPMI1640 或TC199等常用培养基,加适量小牛血清和PHA(植物血球凝集 素),夏季操作时最好加适量青、链霉素,调pH值为7.0~7.2左右,4℃条件下保存6个月 。检测时,以500IU/ml肝素湿润注射器,抽取患者静脉血1.5~2ml(或取耳垂、指尖或足 跟等处微量血),每份培养基(5ml)内种血0.2~0.3ml,37℃温箱内静置培养72小时,收获 前3~4小时加秋水仙素(或秋水仙胺),最终浓度为0.02μg/ml,使细胞分裂终止于中期。 取出培养物行离心、低渗和固定等一系列细胞学操作获得染色体标本,以Giemsa染色后镜检 ,可作染色体标本的普通分析。现各实验室一般将染色体行G显带或必要的其他多种显带, 以较精确地对染色体标本进行分析。
  
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