1) BPI 23 Fcγ1 fusion protein
BPI23-Fcγ1融合蛋白
2) fusion protein BPI23-haFGF
融合蛋白BPI23-haFGF
1.
After purification by affinity chromatography and concentration by lyophilization,the biological functions of fusion protein BPI23-haFGF with bacteria,NIH3T3 cells and mice scalded model were analyzed.
方法将已构建的酵母重组表达菌株pPICZαA-BPI23-haF-GF/X-33进行甲醇诱导表达,通过亲和层析纯化和冻干浓缩后,观察融合蛋白BPI23-haFGF对细菌、NIH3T3细胞以及小鼠烫伤模型的作用。
3) BPI 23-Fcγ1 recombinant bactericidal protein
BPI23-Fcγ1重组抗菌蛋白
4) fusion protein
融合蛋白
1.
Molecular design and characterization of recombinant fusion protein of human IFNα_(2a)-THYα_1;
重组人干扰素α_(2a)和胸腺肽α_1融合蛋白的分子设计及其基因表达产物的鉴定
2.
Expression and purification of glycerol dehydratase β-subunit in E.coli as fusion protein;
甘油脱水酶β亚基融合蛋白在大肠杆菌中的表达和纯化
3.
Pro-apoptotic effect of recombinant anti-HER2 fusion protein ScFv/tBid gene on osteosarcoma E10 cells;
重组抗HER2融合蛋白基因ScFv/tBid对骨肉瘤E10细胞的促凋亡作用
5) Fusion proteins
融合蛋白
1.
Separation and purification of recombinant fusion proteins of IFNα_(2b)-THYα_1;
重组人干扰素α_(2b)和胸腺肽α_1融合蛋白的分离纯化
2.
After induction by isopropyl β-D-1-thiogalactopyranoside(IPTG),the observed yield of crude fusion proteins was up to 260 mg/L.
将构建的含有胸腺肽α1融合蛋白的编码基因插入表达质粒pET28a(+)中,并在大肠杆菌BL21中进行表达,该菌经IPTG诱导融合蛋白高表达后,离心收集菌体,超声波破碎,包含体裂解,目标蛋白质的体外变性复性后,经DEAE-Sepharose Fast Flow阴离子交换层析纯化,每升菌液中可获得260 mg融合蛋白。
3.
Objective: To express the fusion proteins containing VP3 and Tat-PTD (protein transduction domain) proteins, and then purify and dialysis on this fusion proteins.
目的:原核表达VP3与Tat-PTD(protein transduction domain)的融合蛋白,并纯化此融合蛋白,观察Tat-PTD介导VP3进入胃癌细胞SGC7901的透膜效应及VP3在肿瘤细胞中的定位,检测融合蛋白诱导细胞凋亡情况。
6) recombinant protein
融合蛋白
1.
Objective To explore the reasons for the low intracellular transduction efficiency of a previously constructed His-TAT-Flag recombinant protein and establish a more efficient transduction system.
目的探索本室构建的pET14b-His-TAT-Flag重组载体表达的融合蛋白在细胞中转导效率低的原因,建立高效的细胞内转导系统。
2.
The purified recombinant protein was analyzed by SDS-PAGE and Western blotting,the results showed that the CP fragment was highly expressed.
重组蛋白经过Ni+-NTA纯化后,SDS-PAGE和Western blotting分析结果表明,TRSV-CP基因在大肠杆菌中获得了高效表达,产生的融合蛋白分子质量约为34 ku,并具有免疫学活性。
3.
A recombinant protein with about 29kD was expressed stably in the form of insoluble inclusion bodies after IPTG induction.
PCR扩增拟南芥MnSODcDNA的保守区段,构建pET-SOD重组质粒,转化大肠杆菌JM109穴DE3雪,IPTG诱导融合蛋白高效表达;经检测表达产物占菌体总蛋白的69%,且以不溶的包涵体形式存在;表达产物变性后经Ni-NTASuperflow亲和柱分离纯化,得到相对分子质量约为29000的PAGE纯蛋白。
补充资料：fc 23 (fluorocarbon)
CAS：75-46-7
分子式：CHF3
分子质量：70.01
中文名称：三氟甲烷
R23
氟仿
英文名称：trifluoro-Methane
arcton 1
carbon trifluoride
fc 23 (fluorocarbon)
fluoroform
fluoryl
freon 23
freon f-23
freon r 23
性状描述：该品为无色不燃气体。沸点-82.1℃。
用途：致冷剂、灭火剂替代品。
分子式：CHF3
分子质量：70.01
中文名称：三氟甲烷
R23
氟仿
英文名称：trifluoro-Methane
arcton 1
carbon trifluoride
fc 23 (fluorocarbon)
fluoroform
fluoryl
freon 23
freon f-23
freon r 23
性状描述：该品为无色不燃气体。沸点-82.1℃。
用途：致冷剂、灭火剂替代品。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条