3) whole-cell PCR
全细胞PCR
1.
Investigation of algae pollution in Xiliu Lake and identification of toxic cyanobacteria by whole-cell PCR;
西流湖水体藻类污染现状和产毒蓝藻的全细胞PCR检测
4) Single cell PCR
单细胞PCR
1.
The single cell PCR method, which has been developed recently, has many advantages in HAB studies, especially for those species which are difficult to culture in the laboratory.
其中,单细胞PCR方法是对难以室内培养的有害赤潮藻种进行遗传特征研究的一项重要技术。
5) whole cell PCR
全细胞PCR法
1.
In this paper,three new detection methods,whole cell PCR,DNA prole and reporter gene cell receptor are discussed with the aim of detecting the minute amount of red tide toxin and harmful organism to prevent them from enteri.
对利用分子生物学技术对赤潮毒素进行检测的几种方法———全细胞PCR法、DNA探针法及信使基因细胞受体法进行了讨论。
6) single-cell RT-PCR
单细胞RT-PCR
1.
Methods: Human neutrophils were isolated and purified from volunteers,total RNA was extracted and a regular RT-PCR aiming at IL-8Rβ mRNA was performed to ascertain its expression profile in human neutrophils and optimize the reaction conditions for the following single-cell RT-PCR procedures.
方法:采用倍比稀释相对定量单细胞RT-PCR方法分离纯化人外周血中性粒细胞并提取总RNA,针对IL-8RβmRNA设计引物,进行RT-PCR确认IL-8Rβ在中性粒细胞中的基因表达并优化反应条件。
补充资料：精液内细胞个别不同类型细胞检查
精液内细胞个别不同类型细胞检查
诊法。射精液的个别细胞形态的分类测定，是精液分析要取得的参数之一。 取已用瑞氏染色法或吉姆染色法染好的精液涂片，在光镜下计数100个头部卵圆，中段形子 、尾子蜷曲的正常精子，同时在一个视野内，计数欲测的不成熟精子或其他形态的细胞。按 下列公式计算：C=〓〓N×S〓〓100〓〓(式中：C——欲测得的细胞；N—— 在计数 100个精子时同一视野下的细胞数；S——1ml精液中的精子数)。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条