1) bovine fetal fibroblasts
牛胎儿成纤维细胞
1.
pEGFP-C1 eucaryon expression vector was successfully transfected by liposome into bovine fetal fibroblasts.
通过脂质体(FuGENE-6)介导,将真核表达载体pEGFP-C1成功导入体外培养的牛胎儿成纤维细胞,探讨影响外源基因转染效率的参数,如DNA和脂质体的用量、转染的细胞数量以及细胞暴露于DNA与脂质体复合物的时间长度。
2.
We constructed a bovine Prnp promoter-less targeting vector BoPrPneo,then transfected the linearized vector into the bovine fetal fibroblasts BFF through electroporation.
采用启动子捕获策略,构建了牛Prnp启动子缺陷型打靶载体BoPrneo,线性化后,再通过电穿孔转染牛胎儿成纤维细胞BFF,用250μg/mL G418进行药物筛选,共得到99个药物抗性细胞克隆。
2) buffalo fetal fibroblasts
水牛胎儿成纤维细胞
1.
Primary investigation on transfection foreign genes of buffalo fetal fibroblasts;
水牛胎儿成纤维细胞转染外源基因的初步研究
3) Fetal fibroblasts
胎儿成纤维细胞
1.
Dissociation culture and subculturing of porcine cumulus cells and fetal fibroblasts;
猪卵丘细胞和胎儿成纤维细胞的分离培养及传代
2.
The development of nuclear transfer(NT)embryos derived from adult ear fibroblasts, fetal fibroblasts, fetal skin cells and ovary granulosa cells were compared.
9%),均显著低于胎儿成纤维细胞(64。
4) fetal fibroblast
胎儿成纤维细胞
1.
The repetitive ITS(internal transcribed spacers) between rDNA genes were used as the target loci for gene targeting to multiple loci in cow fetal fibroblasts.
利用奶牛rDNA基因间的ITS重复序列作为靶位点,对奶牛胎儿成纤维细胞进行多位点基因打靶,建立以重复序列为靶位点的多位点基因打靶技术,并为克隆定点转基因奶牛提供核供体。
2.
To evaluate the feasibility of gene targeting by homologous recombination in goat fetal fibroblasts, a goat β- casein gene targeting vector pTarget1 was constructed.
为评估在山羊胎儿成纤维细胞中通过同源重组进行基因打靶的可行性,构建打靶载体pTarget1,分别以山羊β 酪蛋白基因的5′、3′侧翼区为2条同源臂,中间插入正筛选基因(Neor),外侧插入负筛选基因(HSV tk)。
3.
The results are as fallows:Experiment 1: Seven fetal fibroblast cell lines were set up from 7 fetuses of purebred England large white pigs at 40 d of gestation by using explant-seeding method.
本研究利用体细胞核移植技术首次成功生产了转ω-3脂肪酸去饱和酶基因(sFat-1)克隆猪,结果如下:实验一:采用组织块培养法,建立了7个38日胎龄的纯种英系大白猪胎儿成纤维细胞系;细胞接触抑制2 d后,经流式细胞术检测发现, 80%以上的细胞处于G0/G1期。
5) embryonic fibroblasts
胎儿成纤维细胞
1.
Isolation and culturing in vitro of yak embryonic fibroblasts;
牦牛胎儿成纤维细胞的分离培养
2.
In our experiments, we studied how the factors affected the transfaction efficiency and what effects the liposome method took on sheep embryonic fibroblasts cells in order to increase transfaction efficiency and grand for sheep biology reactor.
本文以脂质体介导法转染绵羊胎儿成纤维细胞(sFFCs),寻找影响脂质体介导转染sFFCs效率的因素,以期提高转染效率,为转基因绵羊生物反应器打下基础。
6) fetal fibroblast cell
胎儿成纤维细胞
1.
Porcine fetal fibroblast cells,ear fibroblast cells and granulosa cells were used as donor in pig cloning.
以中国农业大学实验用小型猪香猪胎儿成纤维细胞、成年耳成纤维细胞和颗粒细胞3种细胞系为供体细胞进行核移植。
补充资料：成纤维细胞干扰素
分子式：
CAS号：
性质： 一种抗病毒蛋白质，也称为成纤维细胞干扰素。分子量25 000~30 000。为糖蛋白，耐酸。在哺乳动物的结缔组织中是成纤维细胞的产物。从胎儿组织或婴儿色皮中分离得到。干扰素也能从培养细胞制取，培养时使细胞在合适的载体如微珠上形成单层，微珠有很高的表面积，可提高产率。抗病毒作用强。
CAS号：
性质： 一种抗病毒蛋白质，也称为成纤维细胞干扰素。分子量25 000~30 000。为糖蛋白，耐酸。在哺乳动物的结缔组织中是成纤维细胞的产物。从胎儿组织或婴儿色皮中分离得到。干扰素也能从培养细胞制取，培养时使细胞在合适的载体如微珠上形成单层，微珠有很高的表面积，可提高产率。抗病毒作用强。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条