By using Semithin-section method,the structure of female organ before and after fertiltzation, differentiation and distribution of transfer cell early developmental stage of embryo and endosperm were investigated.

The development and ultrastructure of aleurone layer transfer cell in buckwheat( Fagopyrum esculentum Moench)were studied by using electron microscopy.

Inducement of transfer cells and ultrastructural observation of root parts during the formation of Robinia pseudoacacia s root nodule;

The results showed that the hyperplasy of root hair,root deformation and the development of the root epidermal transfer cells were induced by rhizobia.

During seed germination, transfer cells are developed from a part of epidermal cells of the cotyledons with wall ingrowths mostly confined to their outer tangential parts.

The transfection efficiency and intercellular trafficking were certified using Western blot and immunofluorescence.

) endosperm development, the dynamic accumulation of the starch and the changes of cell active were observed by using I2-KI and TTC dyes, and the development of aleurone lay and endosperm transfer cells were studied by using resin embedding and cutting section, which combined optical microscopy with electron microscopy.

We collected maize caryopses of 8 d,10 d,15 d and 20 d after pollination and toke the wanted parts to observe the microstructure and the ultrastructure of endosperm transfer cells by using the methods of resin embedding and the technology of half-thinness(1 000 nm) and ultra-thinness(50~60 nm) slice cutting.