2) Canine distemper virus
犬瘟热病毒
1.
Cloning and expression of canine distemper virus H protein gene fragment;
犬瘟热病毒疫苗株H蛋白基因片段的克隆与表达及表达产物抗原性的初步鉴定
2.
Establishment of an indirect ELISA with a recombinant nucleocapsid protein as antigen for detection of antibodies against canine distemper virus;
犬瘟热病毒重组核蛋白间接ELISA方法的建立
3.
Prokaryotic expression of canine distemper virus nucleocapsid protein and development of indirect ELISA for detection of it s antibody;
犬瘟热病毒核蛋白基因的原核表达及其间接ELISA检测方法的建立
3) canine distemper virus(CDV)
犬瘟热病毒
1.
A RT-nested PCR assay for detecting canine distemper virus(CDV) was established using 2 pairs of primers.
根据犬瘟热病毒(CDV)弱毒株Onderstepoort的核衣壳蛋白(NP)基因序列设计了套式引物,建立了RT-nested PCR检测方法。
2.
The nucleocapsid protein gene(N)located 108 to 1679 situation of Canine distemper virus(CDV)genome and was immunogenicity protein with strong conservatism,so N gene was choosed as aim gene.
核蛋白基因(N)位于犬瘟热病毒基因组的108—1679位置处,是保守性较强的免疫原性蛋白,因此选择N基因作为目的基因,利用酶切、连接等方法构建了含犬瘟热病毒核蛋白基因的穿梭质粒pVAX?E3LPN。
4) CDV
犬瘟热病毒
1.
Cloning and Sequence Analysis of CDV H,F,N Gene;
犬瘟热病毒小熊猫株H、F和N基因的克隆及表达
2.
Expression of CDV F1 Protein in E.coli and Preliminary Use of the Expressed Protein in ELISA;
犬瘟热病毒F1基因的原核表达与初步应用
3.
A SYBR Green I based Real-time RT-PCR assay was developed for detection of canine distemper virus(CDV) using a pair of primers derived from the nucleocapsid protein(NP) gene sequence.
根据GenBank发表的犬瘟热病毒(CDV)核衣壳蛋白(NP)基因序列,设计合成一对引物,建立了基于SYBR GreenⅠReal-time RT-PCR检测CDV核酸。
5) classical swine fever virus
猪瘟病毒
1.
Expression and Immunological Activity of E2 Epitope of Classical swine fever virus;
猪瘟病毒E2蛋白抗原表位的表达与免疫活性研究
2.
Pathogenicity of classical swine fever virus GX-3/98 strain and immunization relativity with attenuated hogcholera virus Chinese C strain;
猪瘟病毒广西地方流行株GX-3/98的致病性及其与兔化弱毒株的免疫相关性
3.
Kinetics of peripheral blood leukocytes apoptosis and changes of CD4~+ and CD8~+ T lymphocyte subpopulations of pigs infected with classical swine fever virus;
猪瘟病毒感染猪外周血白细胞凋亡及CD4~+和CD8~+T淋巴细胞亚群的变化
6) classical swine fever virus(CSFV)
猪瘟病毒
1.
The recombinant retroviral vector pBABE-puro-E2 was constructed by inserting classical swine fever virus(CSFV) Shimen strain E2 gene into pBABE-puro.
利用DNA重组技术将猪瘟病毒石门株囊膜蛋白E2基因定向插入逆转录病毒载体pBABE-puro中,构建重组逆转录病毒载体pBABE-puro-E2。
2.
A classical swine fever virus(CSFV) E2 gene was codon-optimized according to the Sf9 cell codon usage.
根据昆虫细胞密码子偏嗜性,对猪瘟病毒E2基因进行密码子优化,并利用昆虫杆状病毒表达系统进行了表达,表达水平约为野生型E2基因的3倍。
3.
A truncated gene encoding the major antigenic domains of E2 protein of classical swine fever virus(CSFV) was amplified by RT-PCR from the genomic RNA of CSFV C-strain and cloned into pPROEX-HTb expression vector to obtain recombinant pPROEX-tE2.
利用RT-PCR扩增了猪瘟病毒(CSFV)兔化弱毒疫苗株(C株)的E2蛋白主要抗原区(tE2)编码区,并定向克隆到表达载体pPROEX-HTb中,获得重组表达载体pPROEX-tE2,将其转化大肠杆菌DH5α菌株,经IPTG诱导,tE2蛋白获得高效表达;经检测,该重组蛋白能被CSFV C株抗血清识别。
补充资料：瘟头瘟脑
1.垂头丧气﹐精神萎靡不振的样子。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条