Recent advances of six infectious cloning techniques of animal viruses was summarized: plasmidbased transfecant rescue system, PolIPolⅡtranscription rescue system, bacterial artificial chromosome operating system, cosmid operating system, yeast artificial chromosome operating system and bacmid operating system.

In the present study, we conducted and discussed serological investigation, molecular genetic variation, development of inactivated vaccine, coinfection and application of PRRSV infectious cDNA clone.

Full-length sequence analysis of CMV-PGs genomic RNA3 infecting Pinellia ternate and constructing infectious clone;

Establishment of infectious clone system of highly pathogenic H5N1 avian influenza virus A/Duck/Guangxi/53/02;

Construction and virus rescue of an infectious clone of porcine circovirus type 1;

Biological Characteristics of an H5N1 Avian Influenza Virus and Its Infectious Clone;

Immunological characteristics of equine infectious anemia virus derived from infectious molecular clone;

In the study,the LTRs of an infectious molecular clone derived from EIAV donkey leukocyte-attenuated vaccine virus (DLA) were substituted with those of EIAV L strain,a virulent EIAV from which D.

In this study,an NspV site was introduced into the S2 gene of pOK8266, an infectious molecular clone derived from EIAV vaccine virus,and then a 6_histidine tag was introduced into NspV site,generating pOK8266_HIS.