1) histidinol dehydrogenase
L-组氨醇脱氢酶
1.
The Mycobacterium tuberculosis hisD gene encoded histidinol dehydrogenase(MtHDH) was amplified by PCR from Mycobacterium tuberculosis H37Rv strain genomic DNA and cloned into a prokaryotic expression vector pET-28a(+) to construct the recombinant expression plasmid pET-28a-HDH.
coli BL21(DE3)并诱导表达,纯化可溶性的结核分枝杆菌L-组氨醇脱氢酶(HDH),并对其性质进行研究,结果表明:重组结核分枝杆菌L-组氨醇脱氢酶能以L-组氨醇和NAD+为底物催化L-组氨醇生成L-组氨酸;该酶的最适pH值为8。
2) L-GDH
L-谷氨酸脱氢酶
3) recombinant L-sorbose/L-sorbosone dehydrogenase
重组醇醛脱氢酶
1.
By means of ammonium sulfate precipitation, DEAE Sepharose Fast Flow and Q Sepharose High Performance assay, a purified recombinant L-sorbose/L-sorbosone dehydrogenase was obtained from Y517#.
通过超声波破碎菌体、硫酸铵分级沉淀、DEAE Sepharose FastFlow阴离子交换层析,Q Sepharose High Performance柱层析等过程,从Y517#发酵液中分离纯化了重组醇醛脱氢酶,纯化倍数为11倍。
4) L-threonine 3-dehydrogenase
L-苏氨酸3-脱氢酶
5) L-histidinol
L-组氨醇
1.
Purified MtHDH can catalyse L-histidinol to the corresponding amino acid L-histidine.
coli BL21(DE3)并诱导表达,纯化可溶性的结核分枝杆菌L-组氨醇脱氢酶(HDH),并对其性质进行研究,结果表明:重组结核分枝杆菌L-组氨醇脱氢酶能以L-组氨醇和NAD+为底物催化L-组氨醇生成L-组氨酸;该酶的最适pH值为8。
6) Alcohol dehydrogenase
醇脱氢酶
1.
Separation and purification of alcohol dehydrogenase from yeast cell;
从酵母细胞中分离纯化醇脱氢酶
2.
Cloning and sequence analysis of human alcohol dehydrogenase ADH1B*2 allele;
人醇脱氢酶ADH1B*2等位基因的克隆与序列分析
3.
Genetic polymorphisms of alcohol dehydrogenase-2 and alcohol drinking for the susceptibility of the primary hepatocellular carcinoma;
乙醇脱氢酶2基因多态和饮酒习惯与肝癌易感性
补充资料：醇脱氢酶的三极结构
[图]
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条