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1)  double-stranded displacing probe
双链置换探针
1.
The assay combined the high specificity of double-stranded displacing probes with real-time quantitative PCR.
结合荧光双链置换探针的特异性和实时PCR的准确定量能力,建立了一种新颖、准确、价廉且高通量的测定DNA池等位基因频率的方法。
2.
Methods: A pair of primers and a pair of specific double-stranded displacing probes were designed by the sequence characteristics of HPV 16 and 58 subtypes; after real-time fluorescence PCR amplifies, the two subtypes in the same fluorescence channel were distinguished effectively by analysis of non-high-resolution dissociation curve of the HPV.
方法:根据HPV16和HPV58亚型的序列特异性各设计一对引物和一对型特异性双链置换探针,采用实时荧光PCR扩增后,通过非高分辨的融解曲线分析在同一荧光通道内有效区分两种HPVDNA基因亚型。
2)  double-stranded probes
双链探针
3)  Displacing probes
置换探针
1.
The first part is the development of real-time PCR genotyping using allele-specific primers, the second part is the development of another real-time PCR genotyping method using displacing probes.
本论文围绕实时PCR基因分型中的引物基因分型和探针基因分型两个关键技术展开了研究,研究工作主要包括ARMS引物、硫代磷酸化修饰引物以及荧光双链置换探针用于实时PCR基因分型的研究。
4)  fluorophore double chain probe
荧光双链探针
1.
According to the specific sequence of 35S and NOS which have been used in transgenic crops frequently, two pairs of primers and two pairs of corresponding fluorophore double chain probes(FDCP)were designed and synthesized.
根据转基因农作物中最常用的花椰菜花叶病毒启动子 (CaMV 3 5S)和根癌农杆菌终止子(NOS)的序列 ,设计合成了两对不同的引物和相对应的两种荧光双链探针 (FDCP) ,分别建立了多重PCR、应用FDCP的实时荧光PCR同时检测转基因成分 3 5S启动子和NOS终止子的方法 。
2.
According to the specific sequence of 35S and Nos which have been used in transgenic crops frequently, two pairs of primers and two pairs of corresponding fluorophore double chain probes were designed and synthesized for detection of 35S & Nos simultaneously with MF PCR.
根据商品化转基因作物中常用的花椰菜花叶病毒启动子 (CaMV 35S)和根癌农杆菌终止子 (Nos)的序列特点 ,设计并合成了两对引物和相对应的荧光双链探针 ,建立一种应用荧光双链探针的多重荧光PCR同时检测转基因成分 35S启动子和Nos终止子的方法 。
5)  double-stranded fluorescent probes
双链荧光探针
6)  feelerfillingchangingdevice
探针式换纬装置
补充资料:脂肪乳剂(中链及长链复合剂) (10%~20%中/长链脂肪乳剂)
药物名称:脂肪乳剂(中链和长链复合物)

英文名:Fat Emulsion for Injection(MCT/LCT)

别名: 脂肪乳剂(中链及长链复合剂) (10%~20%中/长链脂肪乳剂)
外文名:FatEmulsion forInjection(MCT/LCT)
药理作用及用途:
胃肠外给药,可补充人体热能,避免必需脂肪酸缺乏。具有易于氧化水解、氧化完全、生酮作用和对肝功能影响较少等特点。
适用于创伤以及肝功能不全病人。
用法及用量: 同长链脂肪乳剂。
不良反应: 基本同长链脂肪乳剂。
注意事项: 基本同长链脂肪乳剂。
规格: 注射乳剂 250ml, 500ml



类别:肠内、肠外营养药
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条