Objective:To clarify whether the signal peptide of human nerve growth factor can mediate secretory expression of beta-endorphin and whether there is difference between the efficiency of signal peptides from human and mouse nerve growth factor.

High level secretory expression of human oncostatin M in Pichia pastoris;

Secretory expression of human ScFv against keratin in Pichia pastoris;

Cloning and secretory expression of human carboxypeptidase A1 and its peptide genes;

Construction and secreting expression of eukaryotic expression plasmid carrying human 9 ku-granulysin gene;

The cloning and secreting expression of human interleukin-10 in E.coli;

Construction of a novel secreting expression vectors for Pichia pastoris;

Objective To study the secretion expression of human TALL-1 cDNA in Pichia Pastoris and to obtain recombinant human TALL-1 of good biological activity.

AIM: To achieve secretion expression of mature sCD14 gene in Pichia pastoris and to analyze its LPS binding activity.

T he hsp70 gene was recombined in vitro with fusion secretion expression v ector pMAL-p2 and was transformed into E.

Optimization of ProZZ-EGFP fusion protein secreted expression in E.coli;

Construction of hITF yeast expression vector and secreted expression of hITF in Pichia pastoris;

The secreted expression of HPV16 (Xinjiang strain) E6 in Pichia pastoris.;

Constraction of eukaryotic expression vector of human Cu/Zn-SOD and secretive expression in S.cerevisiae;

The secretive expression of human angiogenesis inhibitor endostatin in yeast Pichia pastoris;

In order to promote the secretive expression of the analog of exendin-4 in recombinant Pichia pastoris,we adopted two strategies: one was to express the multi-copy recombinant protein in tandem and the other was to introduce spacer peptides which contain several amino acids between secretive(sequence) and the N terminal of the recombinant protein.