Human dermal fibroblasts as a feeder layer promote the growth of human keratinocytes;

Development of a G418-resistant feeder layer of embryonic stem cell of mice;

Establishment and screening of feeder layer culture system for mouse embryonic stem cell;

Mitomycin C was used to pretreat MEF for feeder layers of embryonic stem cell and MEF was digested for donor cells of nuclear transfer.

Objective To perform the proteome analysis of conditioned medium prepared from mouse embryonic fibroblast feeder layers by 2-dimensional (2D) electrophoresis and mass spectrometry and to find out the possible differentiation-inhibitory factor in conditioned medium.

To overcome these problems, feeder layers originating from human fetal and adult tissues (including human fetal skin fibroblasts, fallopian-tube epithelial cells et al) have been tested.

Study on preparation of the MEF feeder layer;

There are many factors on porcine PGC proliferation such as serum,feeder,growth factors,the time of passage and some ambiguous problems.

When ES cells were seeded and grown on feeder or 0.

Human postnatal foreskin fibroblasts as feeder cells to support human embryonic stem cell growth;

This study was conducted to research parthenogenetic activation of mouse oocytes and the development capabilities and attachment on the feeder cells of parthenogenetic embryos.