1) recombinant L-sorbose/L-sorbosone dehydrogenase
重组醇醛脱氢酶
1.
By means of ammonium sulfate precipitation, DEAE Sepharose Fast Flow and Q Sepharose High Performance assay, a purified recombinant L-sorbose/L-sorbosone dehydrogenase was obtained from Y517#.
通过超声波破碎菌体、硫酸铵分级沉淀、DEAE Sepharose FastFlow阴离子交换层析,Q Sepharose High Performance柱层析等过程,从Y517#发酵液中分离纯化了重组醇醛脱氢酶,纯化倍数为11倍。
2) Aldehyde/alcohol dehydrogenase
醛/醇脱氢酶
3) alcohol/aldehyde dehydrogenase
醇醛脱氢酶
1.
vulgare SCB329 and the gene fragment aadh encoding alcohol/aldehyde dehydrogenase was amplified by PCR using SCB329 genome as template.
方法:根据酮古龙酸菌SCB329株基因组序列设计引物,通过PCR从SCB329株基因组中扩增醇醛脱氢酶基因aadh;构建载体pBMP3-aadh并在大肠杆菌中表达,经活性染色、体外转化反应等方法考察表达产物的活性。
4) histidinol dehydrogenase
L-组氨醇脱氢酶
1.
The Mycobacterium tuberculosis hisD gene encoded histidinol dehydrogenase(MtHDH) was amplified by PCR from Mycobacterium tuberculosis H37Rv strain genomic DNA and cloned into a prokaryotic expression vector pET-28a(+) to construct the recombinant expression plasmid pET-28a-HDH.
coli BL21(DE3)并诱导表达,纯化可溶性的结核分枝杆菌L-组氨醇脱氢酶(HDH),并对其性质进行研究,结果表明:重组结核分枝杆菌L-组氨醇脱氢酶能以L-组氨醇和NAD+为底物催化L-组氨醇生成L-组氨酸;该酶的最适pH值为8。
5) Aldehyde dehydrogenase
醛脱氢酶
1.
Aldehyde dehydrogenase 2 prevents rat cardiomyocytes from apoptosis induced by hypoxia;
乙醛脱氢酶2在大鼠心肌缺氧损伤中的抗凋亡作用
2.
On the substrate concentration,temperature and pH,the acetaldehyde dehydrogenase Based kinetics in fresh milk was investigated by a spectrophotometer with aldehyde being substrate.
以新鲜牛奶作为研究对象,利用分光光度计,基于底物浓度、温度和pH值研究以乙醛为底物的乙醛脱氢酶动力学系统。
6) aldehyde-alcohol dehydrogenase
乙醛乙醇脱氢酶基因
1.
Primers design of cloning aldehyde-alcohol dehydrogenase fragment for bacteria B49;
菌B49乙醛乙醇脱氢酶基因克隆中的引物设计
补充资料：醇脱氢酶的三极结构
[图]
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条