1) stability and catastrophe
稳定性和突变
4) stabilization and deformation
稳定和变形
5) saturation site-directed mutagenesis
饱和定点突变
1.
In order to further improve its catalytic capability,two mutant libraries,created by saturation site-directed mutagenesis at the amino acid positions 168 and 435,were screened by using the spectroscopic assay based on indigo at 670 nm.
利用饱和定点突变技术对细胞色素P450 BM-3(A74G/F87V/L188Q)(PT)的168和435氨基酸位点分别进行随机突变,根据其羟基化吲哚生成的靛蓝在670nm处具有特殊吸收峰进行高通量筛选,获得了三个高于亲本酶(PT)的突变酶D168H、D168L和E435T。
6) Site-saturation mutagenesis
定点饱和突变
补充资料:稳定极限和静态稳定储备系数
稳定极限和静态稳定储备系数
stability limit and steady state stability margin
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