1 of pH value,and cysteine as the oxide-shuffling reagent,the denatured lysozyme was most favorably renatured.

The result indicated that 50%～60% of the disulfide bonds of the feather keratin were disrupted during extrusion.

To test the hypothesis that in vitro protein cross-linking could be accomplished in three concerted steps:(1) a change in protein conformation;(2) formation of interchain disulfide bonds;and(3) formation of interchain isopeptide cross-links,we amplified wild and Cys/Ser mutant genes with PCR technique from E.

The influence of disulfide bonds on the conformational changes and activities of refolded phytase, the effect of deglycosylation on the stability of the phytase, the effect of metal on the conformation, activities and stability and inhibition kinetics of substrate, product and product analogues on the phytase was studied using Aspergillus sp.

A novel approach has been proposed to predict the disulfide bonding state of cysteines in proteins by constructing a two-stage classifier combining a first global linear discriminator based on their amino acid composition and a second local support vector machine classifier.

A novel disulfide bond-bearing cyclopeptides has been designed and synthesized by means of air oxidation of bis(S-Acm) which was synthesized through Fmoc solid-phase synthesis.

Novel disulfide bond-bearing cyclopeptides have been designed and synthesized by iodine oxidation of bis (S-Acm) of Boc-Glu-(Leu-Cys-OMe)-Leu-Cys-OMe and Boc-Asp-(Leu-Cys-OMe)-Leu-Cys-OMe in high dilute condition.

The Effect of Trinitrotoluene on Soluble Protein Sulfhydryl Groups and Disulfide Bond of Incubated Rat Lenses;

Methods of disulfide formation in peptide synthesis are reviewed in this paper.

Recombinant single chain insulin (PIP) contains three disulfide bonds.