1) p21 promoter
p21启动子
1.
Objective:To clone p21 promoter and insert it into a luciferase reporter gene vector,and to characterize the promoter activity.
方法:采用PCR技术从人乳腺癌细胞系MCF-7基因组中扩增出p21启动子,插入荧光素酶报告基因载体pGL3-basic中,确定所扩增的DNA序列,在293T细胞中检测其活性。
2) promoter/non-promoter
启动子/非启动子
3) promoter
[英][prə'məʊtə(r)] [美][prə'motɚ]
启动子
1.
A DNA Autonomous Replication Origin with Promoter Function in Yeast;
一个具有启动子功能的酵母DNA自主复制起始区
2.
Construction of recombinant of luciferase promoter reporter gene of endothelial-overexpressed lipopolysaccharide-associated factor 1;
人内皮细胞高表达脂多糖相关因子1启动子荧光素酶报告基因重组体的构建
3.
Effect of Aspirin on humanα_2(Ⅰ) collagen promoter induced by rhCD40L;
rhCD40L及阿司匹林对人I型胶原α_2(I)链启动子活性表达的影响
4) 35s promoter
35s启动子
1.
The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35S promoter heterologous template for the detection of genetically modified maize was developed in this study.
建立了玉米转基因成分中35S启动子非同源模板的竞争定量PCR检测系统。
2.
However, the most generally used consititutive CaMV 35S promoter often fail in some incidences, especially for those genes in male organgenesis and male gametogenesis.
然而,在拟南芥遗传学研究中最常用的花椰菜花叶病毒35S启动子在花器官中,尤其在雄蕊中往往并不能做到持续而强烈地表达目的基因,所以有利用35S启动子无法互补突变体表型,或者观察不到异位表型的事例的报导。
3.
And also it is strong recommended that the biosafe construct should have no any uncertain element and exogenous DNA sequence such as antibiotics gene,35S promoter etc.
本研究以pCAMBIA1300载体为基础,基因操作去除pCAMBIA1300质粒上的潮霉素抗性基因和花椰菜花叶病毒的35S启动子序列,构建了两种只含有水稻蜡质基因启动子引导蜡质基因反义片段的表达载体,p13AWY-1和p13AWY-2。
5) hTERT promoter
hTERT启动子
1.
Changes of biological character of SKOV3 cells transfected with HSV-tk gene under the control of hTERT promoter;
hTERT启动子调控的HSV-tk基因逆转录病毒载体的构建及SKOV3转染细胞的生物学特性观察
2.
Aim: To investigate the effect of ganciclovir and topotecan on SKOV3 cell transfected HSV tk under the control of hTERT promoter.
目的 :探讨更昔洛韦 (GCV)与拓扑替康 (TPT)单独或联合应用对携带有hTERT启动子调控的HSV tk基因的SKOV3细胞生长的影响。
3.
METHODS: The hTERT promoter and TK gene were cloned, andwhich was connected to the PGL3 carrier after enzyme digestion, toconstruct the hTERTp/PGL3、TK/PGL3、hTERTp/TK/PGL3 recombinantcarriers, and then to construct the CMV/TK/PGL3 recombinant carrierswith the similar method and regard CMV a.
方法:克隆hTERT启动子及TK基因,酶切后连接到pGL3载体上,构建hTERTp/pGL3,TK/pGL3,hTERTp/TK/pGL3重组载体。
6) C promoter
Cp启动子
1.
AIM: To construct the recombinant adenovirus carrying fusion suicide gene CDglyTK with the C promoter(Cp),one of the key factors in controlling Epstein-Barr virus latent gene expression,and to investigate if the Cp mediates the expression of CDglyTK in CNE1 cells and kills the cancer cells specifically.
目的:构建含EB病毒Cp启动子的CDglyTK双自杀基因靶向腺病毒载体Ad-Cp-CDglyTK,探讨Cp启动子能否特异性调控自杀基因在转染鼻咽癌细胞中表达及靶向性治疗鼻咽癌的可行性。
补充资料:启动子
分子式:
CAS号:
性质:(一)在DNA分子上,可被RNA聚合酶识别并与之结合的一段序列,位于结构基因的上游,在那里使转录起动。强的启动子可转录出更多的nRNA。因而在重组工作菌中常使用强制的启动子,以期取得更多的表达量。(二)一种致癌因素,在癌变过程的第二阶段或多阶段机制中造成第二阶段(促进阶段);一种辅助致癌物。
CAS号:
性质:(一)在DNA分子上,可被RNA聚合酶识别并与之结合的一段序列,位于结构基因的上游,在那里使转录起动。强的启动子可转录出更多的nRNA。因而在重组工作菌中常使用强制的启动子,以期取得更多的表达量。(二)一种致癌因素,在癌变过程的第二阶段或多阶段机制中造成第二阶段(促进阶段);一种辅助致癌物。
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参考词条