1) in vitro cloning
体外克隆
1.
257 bp upstream seauences and 217 bp coding region sequences of cpcB genes from 6 strains of Arthrospira were obtained by in vitro cloning method,and these sequences were then compared with corresponding sequences from Arthrospira FACHB341.
采用体外克隆技术获得来自节旋藻6个品系的cpcB基因的257 bp的上游序列和217 bp的编码区序列,并和来自FACHB341的cpcB基因序列对应部分进行比较分析。
2) Mutagenegis of clone in vitro
克隆的体外诱变
3) cloning vector
克隆载体
1.
Construction of cloning vector of c-myc-TFF2 fusion gene by PCR based gene assembly;
基因拼装构建c-myc-hTFF2融合基因克隆载体pBS-TFF2
2.
The required fragments were selected with correspording restriction endonuclease and inserted into suitable cloning vector by two steps.
用碱裂解法抽提质粒后，利用相应的限制性内切酶切出目的基因片段，连接到合适的载体上，构建了完整的ＭＤＶＢ抗原基因克隆载体。
3.
A protruding T on 3blunt end of the cloning vector was created by adding dTTP and Taq DNA polymerase to the reaction mixtures.
将克隆载体平端切割后，向反应体系中加入ｄＴＴＰ，用ＴａｑＤＮＡ聚合酶处理载体，使其３－端带上一个凸出的单碱基Ｔ，修饰后的载体可直接与纯化的ＰＣＲ产物连接。
4) receptor clone
受体克隆
1.
Aim Expressing muscle acetylcholin e receptors in mammallian cells using receptor clone technique.
目的 利用受体克隆技术 ,建立骨骼肌细胞乙酰胆碱受体 (m nAChR)的实验模型 ,以便进行各种药理研究。
5) cloning recipient
克隆受体
6) the best chromosome clone
优体克隆
补充资料：肺炎克氏杆菌(Klebsiella pneumoniae),菌体外有一层荚膜
[图]
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条