The primary researches on the amount distributions and groups of purple non-sulfur bacteria in estuary sludge of Xinyang Shihe river were carried out.

Seeded with normal anaerobic granular sludge,mixed sludge(normal anaerobic granular sludge and aerobic activate sludge),river sediments respectively, feeding with synthetic wastewater containing ammonia and nitrite, three similar lab-scale UASB systems were started up.

s: Using river sediments as seed sludge, at the condition of pH 5.

Effects of some major factors of anaerobic fermentative biohydrogen production with organic substrates was evaluated using preheated river sediments as seed sludge The results showed that: at 35 ℃ and initial pH of 65, these sediments could use different sugars including xylose,glucose,fructose ,sucrose,cellobiose,starch as substrates to produce hydrogen.